Prenatal physical activity and diet composition affect the expression of nutrient transporters and mTOR signaling molecules in the human placenta
Prenatal physical activity and diet composition affect the expression of nutrient transporters and mTOR signaling molecules in the human placenta
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DOI：
10.1016/j.placenta.2014.11.015
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发表时间：
2015-02-01
期刊：
影响因子：
3.8
通讯作者：
Adamo, K. B.
中科院分区：
文献类型：
--
作者：
Brett, K. E.;Ferraro, Z. M.;Adamo, K. B.
简介：充足的营养输送到胎儿是必不可少的最佳生长。产前体力活动水平和饮食质量的差异影响母体能量平衡，这些因素可能会改变胎盘营养物质的转运。我们调查了符合体力活动指南和母亲饮食质量对16例足月妊娠胎盘中涉及脂肪酸、氨基酸和葡萄糖转运的基因表达以及哺乳动物雷帕霉素靶蛋白（mTOR）和胰岛素信号传导的相关性。用加速度计直接测量体力活动，用24小时饮食回忆评估饮食组成，用定制的聚合酶链反应（PCR）阵列检测基因表达。符合体力活动指南的女性脂肪酸转运蛋白4（FATP 4），胰岛素样生长因子1（IGF 1），和AMP活化蛋白激酶（AMPK）的β非催化亚基，以及较高的SNAT2表达。总糖摄入量与葡萄糖转运蛋白1（GLUT 1）之间存在强正相关（r = 0.897，p = 0.000，n = 12），总糖与mTOR和IGF 1表达之间存在负相关。来自蛋白质的总热量百分比与胰岛素样生长因子1受体（IGF1R）呈负相关（r = 0.605，p = 0.028，n = 13）。讨论：母体体力活动和饮食组成的变化改变了参与脂肪酸、氨基酸和葡萄糖转运以及mTOR信号传导的基因表达。未来对胎盘营养转运的研究应包括对母体PA和饮食习惯的直接测量，以帮助消除混杂因素。(C)2014爱思唯尔有限公司版权所有。
Introduction: Adequate nutrient delivery to the fetus is essential for optimal growth. Differences in prenatal physical activity level and diet quality influence maternal energy balance and these factors may alter placental nutrient transport. We investigated the associations between meeting physical activity guidelines and the quality of maternal diet on the expression of genes involved in fatty acid, amino acid and glucose transport, and mammalian target of rapamycin (mTOR) and insulin signaling in the placenta from 16 term pregnancies.Methods: Physical activity was directly measured with accelerometry, diet composition was assessed with 24 h dietary recalls, and gene expression was measured with custom polymerase chain reaction (PCR) arrays.Results: Women who met physical activity guidelines had lower gene expression of fatty acid transport protein 4 (FATP4), insulin-like growth factor 1 (IGF1), and the beta non-catalytic subunit of AMP-activated protein kinase (AMPK), and a higher expression of SNAT2. There was a strong positive correlation observed between total sugar intake and glucose transporter 1 (GLUT1)(r = 0.897, p = 0.000, n = 12), and inverse correlations between total sugar and mTOR and IGF1 expression. Percentage of total calories from protein was inversely related to insulin-like growth factor 1 receptor (IGF1R) (r = 0.605, p = 0.028, n = 13).Discussion: Variations in maternal physical activity and diet composition altered the expression of genes involved in fatty acid, amino acid and glucose transport and mTOR signaling. Future research on placental nutrient transport should include direct measures of maternal PA and dietary habits to help eliminate confounding factors. (C) 2014 Elsevier Ltd. All rights reserved.