Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes.
Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes.
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DOI：
10.1021/ac301108q
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发表时间：
2012-06
影响因子：
7.4
通讯作者：
S. Kiwanuka;T. Laurila;J. Frank;A. Esposito;K. B. V. D. Geest;L. Pancheri;D. Stoppa;C. Kaminski
中科院分区：
文献类型：
--
作者：
S. Kiwanuka;T. Laurila;J. Frank;A. Esposito;K. B. V. D. Geest;L. Pancheri;D. Stoppa;C. Kaminski
我们提出了一种用于液体在宽光谱带宽上的灵敏吸收测量的光谱仪。该光谱仪结合了非相干超连续谱光源独特的光谱特性和腔衰荡光谱的优点，这是一种自校准技术。使用定制的雪崩光电二极管阵列进行检测，允许以纳秒的时间分辨率同时测量高达不同光谱分量的衰荡时间。最小检测吸收系数在527 nm处为3.2×10(-6)cm(-1)Hz(-1/2)。我们表明，光谱仪能够记录溶血前后血液痕量水平的光谱差异。
We present a spectrometer for sensitive absorption measurements in liquids across broad spectral bandwidths. The spectrometer combines the unique spectral properties of incoherent supercontinuum light sources with the advantages of cavity ring-down spectroscopy, which is a self-calibrating technique. A custom-built avalanche photodiode array is used for detection, permitting the simultaneous measurement of ring-down times for up to 64 different spectral components at nanosecond temporal resolution. The minimum detectable absorption coefficient was measured to be 3.2 × 10(-6) cm(-1) Hz(-1/2) at 527 nm. We show that the spectrometer is capable of recording spectral differences in trace levels of blood before and after hemolysis.