Wobbling Forth and Drifting Back: The Evolutionary History and Impact of Bacterial tRNA Modifications.
Wobbling Forth and Drifting Back: The Evolutionary History and Impact of Bacterial tRNA Modifications.
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DOI：
10.1093/molbev/msy110
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发表时间：
2018-08-01
影响因子：
10.7
通讯作者：
Agashe D
中科院分区：
文献类型：
--
作者：
Diwan GD;Agashe D
沿着tRNA，修饰反密码子碱基的酶是生命之树翻译的一个关键方面。tRNA修饰延长了摆动配对，允许特定的（“目标”）tRNA识别多个密码子并覆盖其他（“非目标”）tRNA，通常提高翻译效率和准确性。然而，详细的进化历史和tRNA修饰酶的影响尚未分析。通过对1093种细菌中5种tRNA修饰的祖先重建，我们发现大多数修饰是真细菌的祖先，但在许多谱系中反复丢失。大多数修饰丢失与非靶tRNA的进化转变相一致，这通常是由基因组GC和相关密码子使用的偏倚增加或基因组减少驱动的。反过来，tRNA修饰的丢失稳定了其他高度动态的tRNA基因库。因此，我们的工作追溯了细菌tRNA修饰的复杂历史，为它们在细菌翻译进化中的作用提供了第一个明确的证据。
Along with tRNAs, enzymes that modify anticodon bases are a key aspect of translation across the tree of life. tRNA modifications extend wobble pairing, allowing specific (“target”) tRNAs to recognize multiple codons and cover for other (“nontarget”) tRNAs, often improving translation efficiency and accuracy. However, the detailed evolutionary history and impact of tRNA modifying enzymes has not been analyzed. Using ancestral reconstruction of five tRNA modifications across 1093 bacteria, we show that most modifications were ancestral to eubacteria, but were repeatedly lost in many lineages. Most modification losses coincided with evolutionary shifts in nontarget tRNAs, often driven by increased bias in genomic GC and associated codon use, or by genome reduction. In turn, the loss of tRNA modifications stabilized otherwise highly dynamic tRNA gene repertoires. Our work thus traces the complex history of bacterial tRNA modifications, providing the first clear evidence for their role in the evolution of bacterial translation.
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影响因子：
4.5
作者：
Hershberg R;Petrov DA
通讯作者：
Petrov DA
影响因子：
4.5
作者：
Nasvall, S. Joakim;Chen, Peng;Bjork, Glenn R.
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Bjork, Glenn R.
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Grosjean H;Breton M;Sirand-Pugnet P;Tardy F;Thiaucourt F;Citti C;Barré A;Yoshizawa S;Fourmy D;de Crécy-Lagard V;Blanchard A
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3
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Bollback JP
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Bollback JP
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3.5
作者：
Kanaya, S;Yamada, Y;Ikemura, T
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Ikemura, T