Element-specific microtomographic imaging of Drosophila brain stained with high-Z probes.
Element-specific microtomographic imaging of Drosophila brain stained with high-Z probes.
复制标题
用高 Z 探针染色的果蝇大脑的元素特异性显微断层成像。
DOI：
10.1107/s0909049508003725
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发表时间：
2008
影响因子：
2.5
通讯作者：
Yoshio Suzuki
中科院分区：
文献类型：
--
作者：
R. Mizutani;A. Takeuchi;Genta Akamatsu;K. Uesugi;Yoshio Suzuki
应用X射线显微断层摄影术的果蝇成年大脑染色的胶体金和铂化合物。生物组织对硬X射线的透明性使得能够对组织内脏的三维结构进行断层成像可视化。每个高Z元素被可视化为三维结构，从在相应的L（III）吸收边缘的差异吸收系数图像。通过胶体金的特异性吸附，选择性地可视化视叶皮质。通过铂浸渍显示的整个结构允许金染色结构的解剖学分配。选择性染色和特异性可视化的生物组织在微米分辨率应阐明三维细胞组织的理解和应用的生物显微结构。
An application of X-ray microtomography to the Drosophila adult brain stained with colloidal gold and a platinum compound is described. The transparency of biological tissue to hard X-rays enables tomographic visualization of the three-dimensional structure of tissue entrails. Each high-Z element was visualized as a three-dimensional structure from the difference absorption coefficient image at the corresponding L(III) absorption edge. The cortex of the optic lobe was selectively visualized by the specific adsorption of colloidal gold. The entire structure revealed by the platinum impregnation allowed the anatomical assignment of the gold-stained structures. Selective staining and specific visualization of biological tissues at micrometer resolution should elucidate the three-dimensional cellular organization essential for the understanding and application of biological microstructures.
蛋白质稳定金溶胶的非特异性结合是免疫细胞化学中的错误来源。
影响因子：
6.6
作者：
Behnke,O;Ammitzboll,T;Jessen,H;Klokker,M;Nilausen,K;Tranum-Jensen,J;Olsson,L
通讯作者：
Olsson,L