Genes associated with the release of dormant buds in tree peonies (Paeonia suffruticosa)
Genes associated with the release of dormant buds in tree peonies (Paeonia suffruticosa)
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DOI：
10.1007/s11738-008-0184-0
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发表时间：
2008-11-01
影响因子：
2.6
通讯作者：
Zheng, Guosheng
中科院分区：
文献类型：
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作者：
Huang, Xin;Xue, Tongtong;Zheng, Guosheng
构建了牡丹休眠芽释放相关基因的差减cDNA文库。为了鉴定解除休眠芽中高表达的基因，对588个克隆进行差异筛选，然后选择185个克隆进行测序。共获得31个独特的基因，其中只有25个序列在NCBI数据库或拟南芥蛋白质数据库中匹配，6个序列没有匹配。许多不同的基因被鉴定为具有未知或假设的功能，而其他基因被推测具有不同的分子功能。通过北方杂交和半定量RT-PCR技术对筛选出的可能与休眠解除相关的候选基因的表达谱进行了分析。结果表明，这些基因的转录表达与植物的生长调控和胁迫反应有关。这些结果为进一步了解牡丹芽休眠解除的分子机制提供了重要信息。
A subtractive cDNA library was developed to study genes associated with the release of dormant buds in tree peony. To identify genes that are highly expressed in buds released from dormancy, 588 clones were examined by differential screening and then 185 clones were selected to be sequenced. A total of 31 unique genes were obtained, of which only 25 sequences had matches in the NCBI database or Arabidopsis thaliana protein database while 6 sequences with no matches. Many of the different genes were identified as having unknown or hypothetical functions while others were speculated to have different molecular functions. The expression profiles of the selected "candidate" genes which may be associated with dormancy release according to their putative function and previous reports were assessed by northern blot and semi-quantitative RT-PCR. The results indicated that the transcriptional expressions of the isolated genes are related to growth regulation and stress response. Our results provide interesting information for further understanding the molecular mechanism of bud dormancy release in tree peony.