In vitro study on different cell response to spherical hydroxyapatite nanoparticles
In vitro study on different cell response to spherical hydroxyapatite nanoparticles
复制标题
DOI：
10.1177/0885328207081350
复制
发表时间：
2008-07-01
影响因子：
2.9
通讯作者：
Li, Haifeng
中科院分区：
文献类型：
--
作者：
Fu, Qiang;Rahaman, Mohamed N.;Li, Haifeng
羟基磷灰石（Hydroxyapatite， HA）因其良好的生物相容性、生物活性和骨结合性能，被广泛应用于骨缺损的填充和金属假体的涂层。研究表明，微小的透明质酸颗粒会引起炎症反应，尤其是针状颗粒。然而，很少有人关注球形透明质酸纳米颗粒的细胞反应。本研究的目的是对合成的透明质酸纳米颗粒进行化学和物理表征，并研究其体外细胞反应。x射线衍射、电子显微镜、氮气吸附和傅里叶变换红外光谱分析表明，颗粒由碳酸盐取代的透明质酸组成，尺寸为20-40nm，比表面积为75 m(2)/g。L929细胞增殖实验表明，球形透明质酸纳米颗粒比市售透明质酸更具生物相容性。另一方面，U2-OS细胞测试结果表明，球形HA纳米颗粒的抑制率随时间和浓度的增加而增加。72h时半有效抑制浓度（IC50）为50.8 μ g/mL，表明合成的球形纳米晶透明质酸颗粒可作为骨肿瘤切除修复的有效生物材料，且无不良反应。
Hydroxyapatite (HA) is widely used in filling of bone defects and coating on metal parts of prosthetic implants due to its excellent biocompatibility, bioactivity, and bone-bonding properties. It has been demonstrated that micro-sized HA particles cause inflammatory reaction, especially for the needle shaped particles. However, little effort has been concentrated on the cell responses of the spherical HA nanoparticles. The aim of the present work is to chemically and physically characterize the synthesized HA nanoparticles and to investigate the in vitro cell responses. X-ray diffraction, electron microscopy, nitrogen adsorption, and Fourier transform infrared spectroscopy revealed that the particles consisted of nearly spherical crystallites of carbonate-substituted HA with size of 20-40nm and specific surface area of 75 m(2)/g. L929 cell proliferation experiments demonstrate that the spherical HA nanoparticles is more biocompatible than commercially available HA. On the other hand, U2-OS cell test results show that the inhibition rate of the spherical HA nanoparticles increases with time and concentration. The half effective inhibitory concentration (IC50) of the nanoparticles was determined to be 50.8 mu g/mL at 72 h. All these data indicated that the synthesized spherical nanocrystalline HA particles can function as an effective biomaterial for bone tumorectomy repair, while having little adverse effect.