Near infrared Raman spectra of human brain lipids
Near infrared Raman spectra of human brain lipids
复制标题
DOI：
10.1016/j.saa.2004.11.017
复制
发表时间：
2005-05-01
影响因子：
4.4
通讯作者：
Salzer, R
中科院分区：
文献类型：
--
作者：
Krafft, C;Neudert, L;Salzer, R
人脑组织，特别是白色物质，含有高脂质含量。这些脑脂质可分为三个主要类别：中性脂质，包括类固醇胆固醇、磷脂和鞘脂。正常人脑组织中的主要脂质是磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酸、鞘磷脂、半乳糖苷、神经节苷脂、硫苷脂和胆固醇。次要脂质是胆固醇酯和甘油三酯。在从正常脑组织转化为肿瘤的过程中，脂质的组成和浓度以特定的方式发生变化。因此，脂质分析可用作区分正常组织与肿瘤以及确定肿瘤类型和肿瘤分级的诊断参数。拉曼光谱已被建议作为一种分析工具，以检测这些变化，即使在手术中的条件。我们用785 nm激发记录了12种主要和次要脑脂质的拉曼光谱，以确定其光谱指纹，用于定性和定量分析。(c)2004 Elsevier B.V.保留所有权利。
Human brain tissue, in particular white matter, contains high lipid content. These brain lipids can be divided into three principal classes: neutral lipids including the steroid cholesterol, phospholipids and sphingolipids. Major lipids in normal human brain tissue are phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidic acid, sphingomyelin, galactocerebrosides, gangliosides, sulfatides and cholesterol. Minor lipids are cholesterol ester and triacylglycerides. During transformation from normal brain tissue to tumors, composition and concentration of lipids change in a specific way. Therefore, analysis of lipids might be used as a diagnostic parameter to distinguish normal tissue from tumors and to determine the tumor type and tumor grade. Raman spectroscopy has been suggested as an analytical tool to detect these changes even under intra-operative conditions. We recorded Raman spectra of the 12 major and minor brain lipids with 785 nm excitation in order to identify their spectral fingerprints for qualitative and quantitative analyses. (c) 2004 Elsevier B.V. All rights reserved.