Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells.
Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells.
复制标题
DOI：
10.1016/j.fct.2013.05.003
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Jian Song;Mengyao Zhao;Xin Liu;Yuchen Zhu;Xiaosong Hu;Fang Chen
中科院分区：
文献类型：
--
作者：
Jian Song;Mengyao Zhao;Xin Liu;Yuchen Zhu;Xiaosong Hu;Fang Chen
丙烯酰胺（AA）存在于许多煮熟的淀粉类食品中，作为一种可能的致癌物质引起了广泛关注。在本研究中，我们研究了矢车菊素-3-葡萄糖苷（Cy-3-glu）预处理的MDA-MB-231细胞中AA毒性的干预。与AA处理组相比，Cy-3-glu能显著抑制AA诱导的细胞毒性，减少活性氧（ROS）的产生，恢复谷胱甘肽（GSH）的耗竭，降低谷胱甘肽过氧化物酶（GPx）和谷胱甘肽S-转移酶（GST）的活性。Cy-3-glu预处理可促进GPx 1、GST P1和γ-谷氨酰半胱氨酸合成酶（γ-GCS）的表达，抑制细胞色素P450 2 E1（CYP 2 E1）的表达。Cy-3-glu对AA诱导的MDA-MB-231细胞氧化应激具有保护作用。
Acrylamide (AA) occurs in many cooked starchy foods and has caused widespread concern as a possible carcinogen. In the present study, we investigate the intervention of AA toxicity in MDA-MB-231 cells pretreated with cyanidin-3-glucoside (Cy-3-glu). Compared to the cells treated with AA, Cy-3-glu significantly inhibited AA-induced cytotoxicity, reduced reactive oxygen species (ROS) generation, recovered glutathione (GSH) depletion and decreased the activities of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Moreover, the expression of GPx1, GSTP1 and gamma-glutamyl cysteine synthase (γ-GCS) were enhanced, and cytochrome P450 2E1 (CYP2E1) expression was inhibited by the pretreatment of Cy-3-glu. Cy-3-glu presents the protective role against oxidative stress induced by AA in MDA-MB-231 cells.