Magnetite formation by a magnetic bacterium capable of growing aerobically
Magnetite formation by a magnetic bacterium capable of growing aerobically
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DOI：
10.1007/bf00169632
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发表时间：
1991-08
影响因子：
5
通讯作者：
T. Matsunaga;T. Sakaguchi;Fumihiko Tadakoro
中科院分区：
文献类型：
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作者：
T. Matsunaga;T. Sakaguchi;Fumihiko Tadakoro
从淡水沉积物中分离到一株螺旋形磁性细菌，并获得了纯培养物。生长培养基中分别含有琥珀酸盐、硝酸盐和苹果酸铁作为碳源、氮源和铁源。被命名为AMB-1的磁性细菌能够在与空气气氛的自由气体交换中生长。当细胞在琼脂上有氧生长时，存在氧化酶活性，并形成不显示过氧化氢酶活性的白色非磁性集落。当初始细胞浓度为105 cells/ml时，在液体培养中细胞浓度为1.4× 109 cells/ml时，4-5天后细胞生长进入稳定期。超声破碎收获的细胞后，从11培养物中获得2.6 mg细菌磁铁矿。
A helically shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The growth medium contained succinate, nitrate and ferric malate as the carbon, nitrogen and iron sources, respectively. The magnetic bacterium, designated AMB-1, was able to grow in free gaseous exchange with an air atmosphere. When cells were grown aerobically on agar, oxidase activity was present, and white non-magnetic colonies, which did not show catalase activity, were formed. The stationary phase of growth was reached 4–5 days later at a cell concentration of 1.4×109cells/ml in liquid culture when an initial cell concentration of 105cells/ml was employed. After ultrasonic disruption of harvested cells, 2.6 mg bacterial magnetite was obtained from a 11 culture.