Describing directional cell migration with a characteristic directionality time.
Describing directional cell migration with a characteristic directionality time.
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DOI：
10.1371/journal.pone.0127425
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tang JX
中科院分区：
文献类型：
--
作者：
Loosley AJ;O'Brien XM;Reichner JS;Tang JX
许多细胞类型可以通过与外部信号的耦合而使它们的运动方向发生偏差。细胞迁移的速度和迁移路径的方向性等特征可以量化，以提供确定哪些生化和生物力学因素影响细胞定向迁移以及影响程度的指标。为了发挥作用，这些指标必须能够在不同的实验环境中重现。然而，由于它们的数值取决于采样间隔和测量误差等技术参数，大多数是不可重复的。为了解决对可重复度量的需求，我们解析地推导出一个称为方向性时间的度量，即识别运动为方向偏倚所需的最小观察时间。我们证明了相应的拟合函数适用于各种遍历的、有方向偏差的运动。当潜在的动力学特性，如速度或方向偏差不随时间改变时，运动是遍历的。测量非遍流运动的方向性不那么简单，但我们也展示了如何分析这类运动。仿真结果表明了方向性时间测量的鲁棒性及其与测量误差的解耦性。作为一个实际的例子，我们演示了定向时间的测量，一步一步，在有噪声的，非遍历的趋化中性粒细胞的轨迹。由于其固有的普遍性，方向性时间应该有助于表征广泛的运动，包括细胞内运输，细胞运动和动物迁移。
Many cell types can bias their direction of locomotion by coupling to external cues. Characteristics such as how fast a cell migrates and the directedness of its migration path can be quantified to provide metrics that determine which biochemical and biomechanical factors affect directional cell migration, and by how much. To be useful, these metrics must be reproducible from one experimental setting to another. However, most are not reproducible because their numerical values depend on technical parameters like sampling interval and measurement error. To address the need for a reproducible metric, we analytically derive a metric called directionality time, the minimum observation time required to identify motion as directionally biased. We show that the corresponding fit function is applicable to a variety of ergodic, directionally biased motions. A motion is ergodic when the underlying dynamical properties such as speed or directional bias do not change over time. Measuring the directionality of nonergodic motion is less straightforward but we also show how this class of motion can be analyzed. Simulations are used to show the robustness of directionality time measurements and its decoupling from measurement errors. As a practical example, we demonstrate the measurement of directionality time, step-by-step, on noisy, nonergodic trajectories of chemotactic neutrophils. Because of its inherent generality, directionality time ought to be useful for characterizing a broad range of motions including intracellular transport, cell motility, and animal migration.
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