The canine hookworm genome:: Analysis and classification of Ancylostoma caninum survey sequences
The canine hookworm genome:: Analysis and classification of Ancylostoma caninum survey sequences
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DOI：
10.1016/j.molbiopara.2007.11.001
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发表时间：
2008-02-01
影响因子：
1.5
通讯作者：
Mitreva, Makedonka
中科院分区：
文献类型：
--
作者：
Abubucker, Sahar;Martin, John;Mitreva, Makedonka
钩虫感染了近10亿人。犬科动物钩虫是研究人类感染的模型，其基因组信息与功能基因组学和蛋白质组学相结合可以加速钩虫防治的进展。作为全面犬钩虫基因组计划的一步，我们生成了104,000个基因组调查序列（gss），并确定了犬钩虫的基因组大小。gss从基因组中组装成57.6 Mb的独特序列，我们通过分离细胞核的流式细胞术估计该序列为347 +/- 1.2 Mb，远远大于其他Rhabditina物种。在GSS序列中共鉴定出5538个基因，加上EST序列，共鉴定出9113个非冗余的犬盲犬基因。基于基因本体和KEGG关联，提供了70%与其他物种基因同源的基因中的许多基因的功能分类，并鉴定了分泌蛋白和膜结合蛋白。(c) 2007 Elsevier B.V.版权所有
Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a genome that we estimate by flow cytometry of isolated nuclei to be 347 +/- 1.2 Mb, substantially larger than other Rhabditina species. Gene finding identified 5538 genes in the GSS assembly, for a total of 9113 non-redundant A. caninum genes when EST sequences are also considered. Functional classifications of many of the 70% of genes with homology to genes in other species are provided based on gene ontology and KEGG associations and secreted and membrane-bound proteins are also identified. (c) 2007 Elsevier B.V. All rights reserved.