Analysis of microRNA Expression in Liquid-Based Cytology Samples May Be Useful for Primary Lung Cancer Diagnosis.
Analysis of microRNA Expression in Liquid-Based Cytology Samples May Be Useful for Primary Lung Cancer Diagnosis.
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DOI：
10.1093/ajcp/aqaa278
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发表时间：
2021-03
影响因子：
3.5
通讯作者：
Yusuke Araki;K. Arihiro;K. Yamaguchi;S. Sakamoto;Y. Horimasu;T. Masuda;S. Miyamoto;Taku Nakashima-Taku-Nakashi
中科院分区：
文献类型：
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作者：
Yusuke Araki;K. Arihiro;K. Yamaguchi;S. Sakamoto;Y. Horimasu;T. Masuda;S. Miyamoto;Taku Nakashima-Taku-Nakashi
目的：怀疑肺癌的患者常行支气管镜检查；然而，我们有时不能做出明确的诊断，导致额外的侵入性检查。许多研究表明，microRNAs （miRs）在癌症中表达异常。我们检测了从液体细胞学（LBC）样本中提取的4种miRs （miR-21、miR-31、miR-182和miR-183）的诊断价值，并验证了它们是否具有诊断价值。方法收集广岛大学附属医院支气管镜检查时获得的18例手术切除组织标本和136例LBC标本。我们从这些样本中提取RNA，并通过逆转录-定量聚合酶链反应比较4个miRs的表达。结果我们证实，这4个miRs在癌组织中的表达明显高于癌旁正常组织。我们在136个细胞学样本中检测了125个（癌症病例83例，非癌症病例42例）中这些miRs的表达。4种miRs在肺癌患者中的表达明显高于非肺癌患者。在被判定为良性或不确定的样本中，肺癌患者的这些miRs水平也显著高于非肺癌患者。结论LBC中miR的表达分析可能有助于原发性肺癌的诊断。
OBJECTIVES Bronchoscopy is frequently performed for patients suspected of having lung cancer; however, we sometimes fail to make a definitive diagnosis, resulting in additional invasive testing. Many studies indicate that microRNAs (miRs) are abnormally expressed in cancers. We examined the diagnostic value of 4 miRs (miR-21, miR-31, miR-182, and miR-183) extracted from liquid-based cytology (LBC) samples and validated whether they were diagnostically useful. METHODS We collected 18 surgically resected tissue samples and 136 LBC specimens obtained during bronchoscopic examination at Hiroshima University Hospital. We extracted RNA from these samples and compared the expression of 4 miRs by reverse transcription-quantitative polymerase chain reaction. RESULTS We confirmed that expression of the 4 miRs was significantly higher in cancer tissues than in tumor-adjacent normal tissues. We examined the expression of these miRs in 125 (cancer cases, 83; noncancer cases, 42) of 136 cytologic samples. Expression of all 4 miRs was significantly higher in patients with lung cancer than in those without lung cancer. Among samples judged as benign or indeterminate, levels of these miRs were also significantly higher in patients with lung cancer than in those without lung cancer. CONCLUSIONS The analysis of miR expression in LBC samples might be helpful for primary lung cancer diagnosis.