Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development
Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development
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暴露于多氯联苯的斑马鱼胚胎的肽组学分析及其对眼睛发育的影响
DOI：
10.1016/j.ecoenv.2019.03.015
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发表时间：
2019
影响因子：
6.8
通讯作者：
Meiling Tong
中科院分区：
文献类型：
--
作者：
Yue Jiang;Shuchun Zhang;Xin Zhang;Nan Li;Qinyu Zhang;Xirong Guo;Xia Chi;Meiling Tong
多氯联苯（PCBs）是一类持久性有机污染物，与儿童眼发育异常密切相关。然而，关于多肽在多氯联苯诱导的眼部发育不良中的作用知之甚少。为了表征PCB暴露对参与眼部系统发育的肽的性质，我们使用液相色谱-质谱/质谱（LC-MS/MS）检测正常和PCB暴露的斑马鱼胚胎之间肽的差异表达。共分析了7900个肽，其中90个差异表达，29个上调，61个下调。这些肽使用独创性途径分析（IPA）和基因本体论（GO）分析，以探讨它们在眼睛发育中的作用。这项研究确定了18个与多氯联苯暴露组的视神经和眼系统的发展相关的肽，以及10个位于其前体蛋白功能域的肽。这些肽为治疗多氯联苯引起的眼发育不良提供了潜在的生物标志物，并可能有助于我们了解有机污染物引起眼发育异常的机制。
Polychlorinated biphenyls (PCBs), a class of persistent organic pollutant, are closely related to abnormal eye development in children. However, little is known regarding the role of peptides in the development of PCBinduced ocular dysplasia. To characterize the nature of PCB exposure on peptides involved in the development of the ocular system, we used liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) to detect differential expression of peptides between normal and PCB-exposed zebrafish embryos. A total of 7900 peptides.were analyzed, 90 of which were differentially expressed, with 29 being up-regulated and 61 down-regulated. These peptides were investigated using ingenuity pathway analysis (IPA) and gene ontology (GO) analysis to explore their role in eye development. This study identified 18 peptides associated with the development of the optic nerve and ocular system in the PCB-exposure group, as well as 10 peptides that are located in the functional domain of their precursor proteins. These peptides provide potential biomarkers for the treatment of ocular dysplasia caused by PCBs and may help us understand the mechanism of abnormal eye development caused by organic pollutants.