Acquisition session length modulates consolidation effects produced by 5-HT2C ligands in a mouse autoshaping-operant procedure.
Acquisition session length modulates consolidation effects produced by 5-HT2C ligands in a mouse autoshaping-operant procedure.
复制标题
采集会话长度调节了5-HT2C配体在鼠标自动扎装手术过程中产生的巩固效应。
DOI：
10.1097/fbp.0b013e328337bde7
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发表时间：
2010-03
影响因子：
1.6
通讯作者：
Foley JJ
中科院分区：
文献类型：
--
作者：
Walker EA;Foley JJ
虽然神经递质5-羟色胺(5-羟色胺，5-羟色胺)被认为是学习和记忆的媒介，但5-羟色胺受体在啮齿动物中的具体作用还需要进一步研究。在这项研究中，不同相对内在有效性的5-HT2C受体配体在一种名为Autoshaping-OPERANT的小鼠学习和记忆模型中进行了测试。在第一天，小鼠被放置在实验室中，并按照不同的间隔时间表呈现一种音调。S的音调一直开着，直到出现戳鼻子的反应，产生了一个带有确保溶液的勺子。然后给小鼠注射生理盐水、MK212(全激动剂)、间氯苯基哌嗪(部分激动剂)、米安色林和SB206 553(反向激动剂)，以及甲基丝氨酸和(+)-2-溴麦角酸二乙胺(+)-酒石酸氢(中性拮抗剂)。每种化合物在第1天的1或2小时采集会话后注入，以研究采集会话长度对巩固的作用。第一天注射5-HT2C反向激动剂米安色林在第二天产生比任何其他测试药物更大的自形操纵子反应的恢复损害。此外，将获取过程的长度减少到1h显著增加了自动整形任务的难度，进一步调节了所测试的5-HT2C配体产生的巩固效应。
Although the neurotransmitter, 5–hydroxytryptamine (serotonin, 5–HT), has been implicated as a mediator of learning and memory, the specific role of 5–HT receptors in rodents requires further delineation. In this study, 5–HT2C receptor ligands of varying relative intrinsic efficacies were tested in a mouse learning and memory model called autoshaping-operant. On day 1, mice were placed in experimental chambers and presented with a tone on a variable interval schedule. The tone remained on for 6 s or until a nose-poke response occurred to produce a dipper with Ensure solution. Mice were then injected with saline, MK212 (full agonist), m-chlorophenylpiperazine (partial agonist), mianserin, and SB206 553 (inverse agonists), and methysergide and (+)-2-bromo lysergic acid diethylamide (+)-hydrogen tartrate (neutral antagonists). Each compound was injected after either 1 or 2–h acquisition sessions on day 1 to investigate the role of acquisition session length on consolidation. Day 1 injection of the 5–HT2C inverse agonist mianserin produced greater retrieval impairments of the autoshaped operant response on day 2 than any other agent tested. Furthermore, decreasing the length of the acquisition session to 1 h significantly increased the difficulty of the autoshaping task further modulating the consolidation effects produced by the 5–HT2C ligands tested.