Disrupted tongue microbiota and detection of nonindigenous bacteria on the day of allogeneic hematopoietic stem cell transplantation
Disrupted tongue microbiota and detection of nonindigenous bacteria on the day of allogeneic hematopoietic stem cell transplantation
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DOI：
10.1371/journal.ppat.1008348
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发表时间：
2020-03-01
期刊：
影响因子：
6.7
通讯作者：
Yamashita, Yoshihisa
中科院分区：
文献类型：
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作者：
Oku, Saori;Takeshita, Toru;Yamashita, Yoshihisa
作者总结异基因造血干细胞移植（allo-HSCT）受者需要接受强化化疗、放疗和抗生素治疗，这可能会影响肠道和口腔微生物群。我们采用了全长16 S rRNA基因测序分析与高分类分辨率使用第三代测序仪，PacBio Sequel，并确定了舌头微生物群的allo-HSCT受体的细菌组成后，调理方案。这种全面的分子方法鉴定了口腔中不常见的34个分类群，占每种舌头微生物群的0-99.4%（中位数为0.27%）。其中，溶血性葡萄球菌和皮氏罗尔斯通氏菌在allo-HSCT受者中经常被发现，并且它们的检测与随访期间较高的死亡风险显著相关。这些结果表明，应密切关注异基因造血干细胞移植受者口腔微生物群破坏的细菌组成。强化化疗、放疗和抗生素引起的肠道微生物群破坏可能导致严重的肠道移植物抗宿主病和感染并发症的发生，导致异基因造血干细胞移植（allo-HSCT）受者的预后较差。虽然口腔中也密集地定植有本土微生物，但allo-HSCT接受者中的细菌组成仍不清楚。我们确定了45名血液病患者在移植当天的舌微生物群组成，并将其与164名社区居民进行了比较。16 S rRNA基因序列的V1-V2区域表明，allo-HSCT接受者的微生物群与社区居住的成年人相比具有较少的多样性和独特性。全长16 S rRNA基因序列在allo-HSCT接受者的微生物群中鉴定了146个细菌分类群，其中34个细菌分类群与保存在扩展的人类口腔微生物组数据库中的主要栖息在口腔中的细菌不对应。值得注意的是，溶血葡萄球菌和/或皮氏罗尔斯通氏菌的检测与随访期间较高的死亡风险显著相关。这些结果表明，在移植当天，allo-HSCT受体的口腔被破坏的微生物群定殖，并表明检测特定的非土著分类群可能是移植结果的预测因子。
Author summaryAllogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients are subjected to intensive chemotherapy, irradiation and antibiotics which could affect the intestinal as well as oral microbiota. We employed full-length 16S rRNA gene sequencing analysis with high taxonomic resolution using a third-generation sequencer, PacBio Sequel, and determined the bacterial composition of the tongue microbiota of allo-HSCT recipients after conditioning regimens. This comprehensive molecular approach identified 34 taxa uncommon in the oral cavity, which constituted 0-99.4% (median, 0.27%) of each tongue microbiota. Of them, Staphylococcus haemolyticus and Ralstonia pickettii were frequently found in allo-HSCT recipients, and their detection was significantly associated with a higher risk of mortality during the follow-up period. These results suggest that careful attention should be given to the bacterial composition of the disrupted oral microbiota in allo-HSCT recipients.Disruption of the intestinal microbiota caused by intensive chemotherapy, irradiation and antibiotics can result in development of severe gut graft-versus-host disease and infectious complications, leading to poorer outcomes among allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Although the oral cavity is also densely colonized by indigenous microorganisms, the bacterial composition in allo-HSCT recipients remains unclear. We determined the tongue microbiota composition of 45 patients with hematological disorders on the day of transplantation and compared them to 164 community-dwelling adults. The V1-V2 regions of the 16S rRNA gene sequences demonstrated that the allo-HSCT recipients had less diverse and distinct microbiota from that of community-dwelling adults. The full-length 16S rRNA gene sequences identified 146 bacterial taxa in the microbiota of allo-HSCT recipients, of which 34 bacterial taxa did not correspond to bacteria primarily inhabiting the oral cavity deposited in the expanded Human Oral Microbiome Database. Notably, the detection of Staphylococcus haemolyticus and/or Ralstonia pickettii was significantly associated with a higher risk of mortality during the follow-up period. These results demonstrate that the oral cavity of allo-HSCT recipients is colonized by a disrupted microbiota on the day of transplantation and suggest that detection of specific nonindigenous taxa could be a predictor of transplant outcome.