FILAMENTOUS FUSION PHAGE - NOVEL EXPRESSION VECTORS THAT DISPLAY CLONED ANTIGENS ON THE VIRION SURFACE
FILAMENTOUS FUSION PHAGE - NOVEL EXPRESSION VECTORS THAT DISPLAY CLONED ANTIGENS ON THE VIRION SURFACE
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DOI：
10.1126/science.4001944
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
SMITH, GP
中科院分区：
文献类型：
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作者：
SMITH, GP
外源DNA片段可以插入到丝状噬菌体基因III中，形成中间带有外源序列的融合蛋白。融合蛋白被结合到病毒粒子中，病毒粒子保持感染性，并以免疫可及的形式展示外来氨基酸。通过与针对外源序列的抗体的亲和力，这些融合噬菌体可以比普通噬菌体丰富1000倍以上。当针对基因产物的抗体可用时，融合噬菌体可能提供一种简单的克隆基因的方法。
Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These fusion phage can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.