Epidural cortical stimulation enhances motor function after sensorimotor cortical infarcts in rats
Epidural cortical stimulation enhances motor function after sensorimotor cortical infarcts in rats
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DOI：
10.1016/j.expneurol.2006.02.131
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发表时间：
2006-08-01
影响因子：
5.3
通讯作者：
Jones, Theresa A.
中科院分区：
文献类型：
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作者：
Adkins, DeAnna L.;Campos, Peter;Jones, Theresa A.
本研究探讨了脑内皮层电刺激（CS）是否改善了运动康复训练的疗效，并改变了缺血性感觉运动皮层（SMC）病变后病变周围皮层的神经元密度和/或细胞增殖。成年大鼠预先训练熟练的达到任务，然后接受部分单侧SMC损伤和电极植入剩余的SMC。10 ~ 14天后，大鼠接受每日伸展训练，同时进行阳极或阴极100 Hz CS或无刺激（NoCS）18天。为了标记新产生的细胞，训练的每三天施用溴脱氧尿苷（BrdU; 50 mg/kg）。与NoCS对照相比，阳极CS和阴极CS都鲁棒地增强了到达性能。与NoCS组相比，阴极CS组的病灶周围皮质中的神经元密度显著增加。在同侧皮质中BrdU标记的细胞密度没有显著的组间差异。Fluoro-Jade-B染色表明，当皮层刺激和康复开始时，梗死区附近的神经元继续退化。这些数据表明，脑内递送CS大大提高了SMC损伤后康复性伸展训练的疗效，并提高了阴极CS可能影响病变周围皮质神经元存活的可能性。(c)2006年爱思唯尔公司All rights reserved.
This study examined whether epidurally delivered cortical electrical stimulation (CS) improves the efficacy of motor rehabilitative training and alters neuronal density and/or cell proliferation in perilesion cortex following ischemic sensorimotor cortex (SMC) lesions. Adult rats were pre-trained on a skilled reaching task and then received partial unilateral SMC lesions and implantation of electrodes over the remaining SMC. Ten to fourteen days later, rats received daily reach training concurrent with anodal or cathodal 100 Hz CS or no stimulation (NoCS) for 18 days. To label newly generated cells, bromodeoxyuridine (BrdU; 50 mg/kg) was administered every third day of training. Both anodal and cathodal CS robustly enhanced reaching performance compared to NoCS controls. Neuronal density in the perilesion cortex was significantly increased in the cathodal CS group compared to the NoCS group. There were no significant group differences in BrdU-Iabeled cell density in ipsilesional cortex. Staining with Fluoro-Jade-B indicated that neurons continue to degenerate near the infarct at the time when cortical stimulation and rehabilitation were initiated. These data indicate that epidurally delivered CS greatly improves the efficacy of rehabilitative reach training following SMC damage and raise the possibility that cathodal CS may influence neuronal survival in perilesion cortex. (c) 2006 Elsevier Inc. All rights reserved.