A Panel of Serum MiRNA Biomarkers for the Diagnosis of Severe to Mild Traumatic Brain Injury in Humans.
A Panel of Serum MiRNA Biomarkers for the Diagnosis of Severe to Mild Traumatic Brain Injury in Humans.
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DOI：
10.1038/srep28148
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发表时间：
2016-06-24
影响因子：
4.6
通讯作者：
Maheshwari RK
中科院分区：
文献类型：
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作者：
Bhomia M;Balakathiresan NS;Wang KK;Papa L;Maheshwari RK
MicroRNAs(MiRNAs)是一种小的内源性RNA分子，由于其稳定性和微量检测，已成为多种疾病的新的血清诊断生物标志物。在本研究中，我们在轻、重度脑损伤患者的血清样本中发现了一种可用于诊断轻、中度脑损伤(MMTBI)的血清miRNA信号。采用MMTBI、重型颅脑损伤(STBI)、骨科创伤和健康对照的人血清标本，用TaqMan实时定量聚合酶链式反应(TaqMan Real Time PCR)进行miRNA图谱分析。将MMTBI、STBI和骨科损伤的实时PCR数据归一化到对照样本，结果显示MMTBI、STBI和骨科损伤组的miRNAs分别上调了39、37和33个。将颅脑损伤组与骨科损伤组进行比较，观察到MMTBI组和STBI组分别有18和20个miRNAs上调。其中，在MMTBI和STBI组中都发现有10个miRNAs的特征。在STBI的脑脊液中验证了这10个miRNAs，发现有4个miRNAs在脑脊液中表达上调。综上所述，我们确定了可用于诊断MMTBI和STBI的10个独特miRNAs的子集。
MicroRNAs (MiRNAs) are small endogenous RNA molecules and have emerged as novel serum diagnostic biomarkers for several diseases due to their stability and detection at minute quantities. In this study, we have identified a serum miRNA signature in human serum samples of mild to severe TBI, which can be used for diagnosis of mild and moderate TBI (MMTBI). Human serum samples of MMTBI, severe TBI (STBI), orthopedic injury and healthy controls were used and miRNA profiling was done using taqman real time PCR. The real time PCR data for the MMTBI, STBI and orthopedic injury was normalized to the control samples which showed upregulation of 39, 37 and 33 miRNAs in MMTBI, STBI and orthopedic injury groups respectively. TBI groups were compared to orthopedic injury group and an up-regulation of 18 and 20 miRNAs in MMTBI and STBI groups was observed. Among these, a signature of 10 miRNAs was found to be present in both MMTBI and STBI groups. These 10 miRNAs were validated in cerebrospinal fluid (CSF) from STBI and four miRNAs were found to be upregulated in CSF. In conclusion, we identified a subset of 10 unique miRNAs which can be used for diagnosis of MMTBI and STBI.