Bacterial Diversity and Community Structure in Two Bornean Nepenthes Species with Differences in Nitrogen Acquisition Strategies
Bacterial Diversity and Community Structure in Two Bornean Nepenthes Species with Differences in Nitrogen Acquisition Strategies
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DOI：
10.1007/s00248-015-0723-3
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发表时间：
2016-01
影响因子：
3.6
通讯作者：
Wiebke Sickel;T. Grafe;I. Meuche;I. Steffan‐Dewenter;A. Keller
中科院分区：
文献类型：
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作者：
Wiebke Sickel;T. Grafe;I. Meuche;I. Steffan‐Dewenter;A. Keller
猪笼草属的食肉植物已经被研究了超过一个世纪，但令人惊讶的是，人们对它与微生物的关系知之甚少。这两种猪笼草（Nepenthes rafflesiana）和猪笼草（Nepenthes hemsleyana）在投手介导的营养源方面有所不同，分别从节肢动物猎物和节肢动物以及蝙蝠粪便中吸收氮。我们预计生活在投手中的细菌群落类似于这种饮食差异。样品取自这两个物种的投手的不同部分（叶，口，内部，外部，消化液）。使用非培养物依赖性高通量扩增子测序确定细菌群落。两种植物叶片、口缘、内外壁细菌丰度和群落结构相似。在消化液中，N组细菌丰度较高。hemsleyanathan in N. rafflesiana.此外，消化液社区的结构变化很大，重复之间的社区组成的菌株特异性差异。除Acidocella属在两个N.莱佛士类客栈与N. rafflesiana，与satellites构成营养的潜在竞争者。群落结构的高度变异可能是由许多生物和非生物因素造成的。固氮细菌存在于这两个研究物种，这可能会提供必要的营养物质的植物在低猎物捕获和/或罕见的遭遇蝙蝠的时候。
Carnivorous plants of the genusNepentheshave been studied for over a century, but surprisingly little is known about associations with microorganisms. The two speciesNepenthes rafflesianaandNepenthes hemsleyanadiffer in their pitcher-mediated nutrient sources, sequestering nitrogen from arthropod prey and arthropods as well as bat faeces, respectively. We expected bacterial communities living in the pitchers to resemble this diet difference. Samples were taken from different parts of the pitchers (leaf, peristome, inside, outside, digestive fluid) of both species. Bacterial communities were determined using culture-independent high-throughput amplicon sequencing. Bacterial richness and community structure were similar in leaves, peristomes, inside and outside walls of both plant species. Regarding digestive fluids, bacterial richness was higher inN. hemsleyanathan inN. rafflesiana. Additionally, digestive fluid communities were highly variable in structure, with strain-specific differences in community composition between replicates. Acidophilic taxa were mostly of low abundance, except the genusAcidocella, which strikingly reached extremely high levels in twoN. rafflesianafluids. InN. hemsleyanafluid, some taxa classified as vertebrate gut symbionts as well as saprophytes were enriched compared toN. rafflesiana, with saprophytes constituting potential competitors for nutrients. The high variation in community structure might be caused by a number of biotic and abiotic factors. Nitrogen-fixing bacteria were present in both study species, which might provide essential nutrients to the plant at times of low prey capture and/or rare encounters with bats.