Mesenchyme specificity in rodent salivary gland development: the response of salivary epithelium to lung mesenchyme in vitro.
Mesenchyme specificity in rodent salivary gland development: the response of salivary epithelium to lung mesenchyme in vitro.
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啮齿动物唾液腺发育中的间充质特异性：体外唾液上皮对肺间充质的反应。
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发表时间：
1974
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通讯作者：
K. Lawson
中科院分区：
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作者：
K. Lawson
肺间充质在体外能够支持唾液腺上皮原基的出芽和细胞分化。在小鼠或大鼠的下颌下腺和腮腺上皮之间未发现反应差异。这一结果还有其他一些特点，与先前的发现相矛盾。（1）肺间充质在支持下颌下腺形态发生方面，其效果在数量上不如下颌下腺间充质。这种差异至少部分归因于下颌下腺上皮无法替代肺上皮来支持肺间充质的生长。（2）当大鼠肺间充质与小鼠下颌下腺上皮重组时，其在数量上比小鼠肺间充质更有效。这可能至少部分是由于小鼠肺更容易被所采用的操作程序损伤。（3）尽管下颌下腺上皮对下颌下腺间充质的反应在琼脂或微孔滤膜（MF）基质上同样良好，但对肺间充质的反应在MF上严重减弱或消失。这种差异是根据下颌下腺或肺间充质支持分支形态发生所需的不同间充质细胞密度来解释的。6天后在肺间充质中形成的唾液腺芽比在唾液腺间充质中更小且排列更紧密。在这些异型重组体中，芽中抗淀粉酶、PAS阳性物质的积累最初加速，并且管状上皮积累糖原。
Lung mesenchyme is able to support budding and cytodifferentiation of salivary epithelial rudiments in vitro . No difference in response was found between submandibular and parotid epithelium from mouse or rat. There are several further features of this result, which is contradictory to previous findings. (1) Lung mesenchyme is quantitatively less effective than submandibular mesenchyme for supporting submandibular morphogenesis. At least part of this difference is attributed to the inability of submandibular epithelium to replace lung epithelium in supporting the growth of lung mesenchyme. (2) Rat lung mesenchyme is quantitatively more effective than mouse lung mesenchyme when recombined with mouse submandibular epithelium. This may be at least partly due to mouse lung being more easily damaged by the procedures used. (3) Whereas the response of submandibular epithelium to submandibular mesenchyme is equally good on an agar or Millipore filter (MF) substratum, the response to lung mesenchyme is severely reduced or eliminated on MF. This difference is interpreted in terms of different mesenchymal cell densities necessary for submandibular or lung mesenchyme to support branching morphogenesis. Salivary buds formed in lung mesenchyme after 6 days are smaller and more closely packed than in salivary mesenchyme. In these heterotypic recombinates, the accumulation of amylase-resistant, PAS-positive material in the buds is initially accelerated and the tubular epithelium accumulates glycogen.