Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
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DOI：
10.3892/ijmm.2014.1943
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发表时间：
2014-12
影响因子：
5.4
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Liu MW;Wang YH;Qian CY;Li H
川芎、赤芍、丹参、红花具有较强的抗炎作用，已被用于治疗炎症性感染性疾病。本实验研究了XBJ对盲肠结扎穿刺（CLP）致脓毒症大鼠肺通透性和肺损伤的影响。对照组和治疗组分别建立CLP脓毒症模型。治疗组在手术前约2 h给予XBJ注射液100 mg/kg。采用逆转录聚合酶链反应（PT-PCR）和western blot检测肺组织中toll相互作用蛋白（Tollip）、白细胞介素-1受体相关激酶1 （IRAK1）、toll样受体4 （TLR4）、核因子-κB65 （NF-κB65）和TNF受体相关因子6 （TRAF6）的表达。采用ELISA法检测支气管肺泡灌洗液（BAL）中肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）、白细胞介素-1 （IL-1）、白细胞介素-4 （IL-4）和白细胞介素-10 （IL-10）水平以及血清细胞间粘附分子1 （ICAM-1）和血管性血友病因子（vWF）水平的变化。测定BAL液中中性粒细胞、白蛋白及总细胞数。组织学分析采用苏木精和伊红（H&E）染色。CLP诱导ALI 24 h后，观察肺通透性、干湿比（W/D）及肺病理评分。结果表明，XBJ上调Tollip表达，抑制IRAK1、TLR4、NF-κβ65、TRAF6活性。此外，与对照组相比，XBJ组中性粒细胞数量和总细胞数量明显减少。XBJ组肺通透性、湿/干重比（W/D）和肺病理评分均显著降低。组织学结果也证实了XBJ对clp诱导的肺部炎症的抑制作用。本研究结果表明，XBJ通过上调Tollip的表达，显著降低clp诱导的肺通透性。XBJ的保护作用提示其在clp诱导的急性肺损伤治疗中的治疗潜力。
Xuebijing (XBJ) is a type of traditional Tibetan medicine, and previous pharmacological studies have shown that the ethanol extract is derived from Chuanxiong, Chishao, Danshen and Honghua. Chuanxiong, Chishao, Danshen and Honghua possesses potent anti-inflammatory activity, and has been used in the treatment of inflammatory infectious diseases. In the present study, we investigated the effects of XBJ on pulmonary permeability and lung injury in cecal ligation and puncture (CLP)-induced sepsis in rats. A CLP sepsis model was established for the control and treatment groups, respectively. Approximately 2 h prior to surgery, an amount of 100 mg/kg XBJ injection was administered to the treatment group. Reverse transcription polymerase chain reaction (PT-PCR) and western blot analysis were used to examine the expression of Toll-interacting protein (Tollip), interleukin-1 receptor-associated kinase 1 (IRAK1), Toll-like receptor 4 (TLR4), nuclear factor-κB65 (NF-κB65) and TNF receptor-associated factor 6 (TRAF6) in lung tissue. ELISA was applied to detect changes of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 (IL-1), interleukin-4 (IL-4) and interleukin-10 (IL-10) levels in bronchoalveolar lavage (BAL) fluid, and intercellular adhesion molecule 1 (ICAM-1) and von Willebrand factor (vWF) in serum. The number of neutrophils, albumin and total cells in the BAL fluid were measured. For histological analysis, hematoxylin and eosin (H&E) stains were evaluated. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were determined following the induction of ALI by CLP for 24 h. The results demonstrated that XBJ upregulated Tollip expression and blocked the activity of IRAK1, TLR4, NF-κβ65 and TRAF6. Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were significantly decreased in the XBJ group. The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation. The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression. The protective effects of XBJ suggest its therapeutic potential in CLP-induced acute lung injury treatment.
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影响因子：
2.1
作者：
Nan, Yong Hai;Park, Il-Seon;Shin, Song Yub
通讯作者：
Shin, Song Yub
DOI：
10.1164/rccm.200804-535oc
发表时间：
2009-01-15
影响因子：
24.7
作者：
Cho, Hye-Youn;Imani, Farhad;Kleeberger, Steven R.
通讯作者：
Kleeberger, Steven R.
影响因子：
4.8
作者：
Katoh, Y;Shiba, Y;Nakayama, K
通讯作者：
Nakayama, K
影响因子：
3.3
作者：
Berthiaume, Y;Folkesson, HG;Matthay, MA
通讯作者：
Matthay, MA
抑制白介素1受体/TOLL样受体信号传导通过剪接的短形式的MyD88抑制是由于其未能募集IRAK-4所致。
影响因子：
15.3
作者：
Burns, K;Janssens, S;Brissoni, B;Olivos, N;Beyaert, R;Tschopp, J
通讯作者：
Tschopp, J