RAPID DETECTION OF SIMULATED BACTEREMIA BY CENTRIFUGATION AND FILTRATION
RAPID DETECTION OF SIMULATED BACTEREMIA BY CENTRIFUGATION AND FILTRATION
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DOI：
10.1128/jcm.16.1.99-102.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
LEFROCK, JL
中科院分区：
文献类型：
--
作者：
HERLICH, MB;SCHELL, RF;LEFROCK, JL
开发了一种离心过滤程序，以加速血液中微生物的回收。用新鲜人全血接种各种需氧和兼性厌氧微生物（3-18个/ml）。将种子血小心地覆盖在Ficoll-Hypaque梯度（密度，1.114 g/ml）上，离心400次。G)室温下45min。整个梯度（血浆，白细胞和Ficoll-Hypaque）被去除并通过0.22-.mu过滤。M膜过滤器。然后将滤光片放在巧克力琼脂上，在35度的温度下孵育。C在含5% CO2的湿空气中。通过过滤和直接培养原始接种物回收的微生物数量之间没有统计学上的显著差异。大多数微生物在过滤后18h内检出。该系统具有极好的敏感性和可忽略的毒性。
A centrifugation-filtration procedure was developed to expedite the recovery of microorganisms from blood. Fresh whole human blood was inoculated with various aerobic and facultatively anaerobic microorganisms (3-18/ml). The seeded blood was carefully overlaid on a Ficoll-Hypaque gradient (density, 1.114 g/ml) and centrifuged (400 .times. g) for 45 min at ambient temperature. The entire gradient (plasma, leukocytes and Ficoll-Hypaque) was removed and filtered through a 0.22-.mu.m membrane filter. The filters were then placed on chocolate agar and incubated at 35.degree. C in humidified air containing 5% CO2. No statistically significant differences were detected between the numbers of microorganisms recovered by filtration and by direct culture of the original inoculum. Most microorganisms were detected within 18 h after filtration. This system has excellent sensitivity and negligible toxicity.