Segmental extracellular and intracellular water distribution and muscle glycogen after 72-h carbohydrate loading using spectroscopic techniques
Segmental extracellular and intracellular water distribution and muscle glycogen after 72-h carbohydrate loading using spectroscopic techniques
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DOI：
10.1152/japplphysiol.00126.2016
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发表时间：
2016-07-01
影响因子：
3.3
通讯作者：
Takahashi, Hideyuki
中科院分区：
文献类型：
--
作者：
Shiose, Keisuke;Yamada, Yosuke;Takahashi, Hideyuki
在碳水化合物负荷期间，体内水分含量增加，因为每1克糖原与2.7-4克水结合。生物电阻抗谱（BIS）可以单独评估整个身体和每个身体部位的细胞外和细胞内水（ECW和ICW分别）。然而，BIS尚未被证明可以检测由碳水化合物负荷引起的体内水分变化。在这里，我们的目的是研究BIS是否有足够的灵敏度来检测体内水分含量的变化，并确定碳水化合物负荷后的部分水分分布。8名受试者食用含有12克碳水化合物的高碳水化合物饮食。Kg体重（-1）。糖原消耗循环运动72小时后第(1)天。采用c -13磁共振波谱法测定肌糖原浓度变化，采用氘稀释技术（TBWD2O）测定全身水（TBW）。采用BIS评估全身（腕-踝）和各身体节段（手臂、躯干和腿部）的ICW和ECW。运动后72 h，肌糖原浓度[72.7 +/- 10.0 (SD) ~ 169.4 +/- 55.9 mmol/kg wet wt, P < 0.001]和TBWD2O （39.3 +/- 3.2 ~ 40.2 +/- 3.0 kg, P < 0.05）分别较基线显著升高。全身BIS在ICW组显著升高（P < 0.05），但在ECW组无显著升高。节段性BIS显示腿部ICW显著升高（P < 0.05），而手臂和躯干ICW无显著升高。我们的研究结果表明，碳水化合物负荷后体内水分的增加可以通过BIS检测到，并且是由特定节段的ICW增加引起的。
Body water content increases during carbohydrate loading because 2.7-4-g water binds each 1 g of glycogen. Bioelectrical impedance spectroscopy (BIS) allows separate assessment of extracellular and intracellular water (ECW and ICW, respectively) in the whole body and each body segment. However, BIS has not been shown to detect changes in body water induced by carbohydrate loading. Here, we aimed to investigate whether BIS had sufficient sensitivity to detect changes in body water content and to determine segmental water distribution after carbohydrate loading. Eight subjects consumed a high-carbohydrate diet containing 12 g carbohydrates.kg body mass(-1) . day(-1) for 72 h after glycogen depletion cycling exercise. Changes in muscle glycogen concentration were measured by C-13-magnetic resonance spectroscopy, and total body water (TBW) was measured by the deuterium dilution technique (TBWD2O). ICW and ECW in the whole body (wrist-to-ankle) and in each body segment (arm, trunk, and leg) were assessed by BIS. Muscle glycogen concentration [72.7 +/- 10.0 (SD) to 169.4 +/- 55.9 mmol/kg wet wt, P < 0.001] and TBWD2O (39.3 +/- 3.2 to 40.2 +/- 3.0 kg, P < 0.05) increased significantly 72 h after exercise compared with baseline, respectively. Whole-body BIS showed significant increases in ICW (P < 0.05), but not in ECW. Segmental BIS showed significant increases in ICW in the legs (P < 0.05), but not in the arms or trunk. Our results suggest that increase in body water after carbohydrate loading can be detected by BIS and is caused by segment-specific increases in ICW.