Comprehensive database and evolutionary dynamics of U12-type introns
Comprehensive database and evolutionary dynamics of U12-type introns
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DOI：
10.1093/nar/gkaa464
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发表时间：
2020-07-27
影响因子：
14.9
通讯作者：
Padgett, Richard A.
中科院分区：
文献类型：
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作者：
Moyer, Devlin C.;Larue, Graham E.;Padgett, Richard A.
在大多数真核前信使RNA和长非编码RNA的核成熟过程中，内含子通过RNA剪接过程被去除。不同类型的内含子被U2型或U12型剪接体切除，剪接体是小的核核糖核蛋白颗粒和相关蛋白质的大复合物。我们创建了intronIC，这是一个将内含子类分配给给定基因组中所有内含子的程序，并将其用于24个真核基因组，以创建内含子注释和同源性数据库（IAOD）。然后，我们使用IAOD中的数据重新审视了关于两类剪接体内含子进化的几个假设，为解释现代基因组中U12型内含子丰度低的类转换模型找到了支持。
During nuclear maturation of most eukaryotic premessenger RNAs and long non-coding RNAs, introns are removed through the process of RNA splicing. Different classes of introns are excised by the U2-type or the U12-type spliceosomes, large complexes of small nuclear ribonucleoprotein particles and associated proteins. We created intronIC, a program for assigning intron class to all introns in a given genome, and used it on 24 eukaryotic genomes to create the Intron Annotation and Orthology Database (IAOD). We then used the data in the IAOD to revisit several hypotheses concerning the evolution of the two classes of spliceosomal introns, finding support for the class conversion model explaining the low abundance of U12-type introns in modern genomes.