CHLOROPHYLL METABOLISM IN HIGHER-PLANTS .7. CHLOROPHYLL DEGRADATION IN SENESCING TOBACCO-LEAVES - PHENOLIC-DEPENDENT PEROXIDATIVE DEGRADATION
CHLOROPHYLL METABOLISM IN HIGHER-PLANTS .7. CHLOROPHYLL DEGRADATION IN SENESCING TOBACCO-LEAVES - PHENOLIC-DEPENDENT PEROXIDATIVE DEGRADATION
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DOI：
10.1139/b87-097
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发表时间：
1987-04-01
期刊：
影响因子：
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通讯作者：
SHIMIZU, S
中科院分区：
文献类型：
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作者：
KATO, M;SHIMIZU, S
烟草的膜(类囊体)和蛋白质(捕光类叶绿素蛋白复合体)结合的叶绿素不仅能被溶解的叶绿素漂白，而且还能被酚-过氧化物酶-H_2O_2体系漂白。老叶的类囊体膜漂白率高于幼叶。从老化的叶片制备的类囊体膜中也观察到了类似的现象。衰老烟叶的过氧化物酶活性、过氧化氢含量和总酚含量较高。衰老叶片对过氧化氢降解的防御机制不如幼叶活跃。这些结果表明，衰老引起的亚细胞环境的恶化通过过氧化物酶催化的反应加速了叶绿素的漂白。
Not only solubilized chlorophyll but also membrane (thylakoid) and protein (light-harvesting chlorophyll-protein complex) bound chlorophyll of tobacco plants [Nicotiana tabacum]was bleached by a phenolic-peroxidase-H2O2 system. The bleaching rate of thylakoid membranes in old leaves was higher than in young leaves. A similar phenomenom was observed in thylakoid membranes prepared from aged leaves. Peroxidase activity, peroxide content, and total phenolic content were high in senescent tobacco leaves. The defence mechanism against peroxide chlorophyll degradation was less active in senescent leaves than in young leaves. These results suggest that deterioration of the subcellar environment induced by senescence accelerates chlorophyll bleaching by a peroxidase-catalyzed reaction.