MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis
MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis
复制标题
拟南芥响应盐胁迫时皮质微管的解聚和重组需要 MAP65-1
DOI：
10.1016/j.plantsci.2017.09.004
复制
发表时间：
2017
期刊：
影响因子：
5.2
通讯作者：
Li Yingzhang
中科院分区：
文献类型：
--
作者：
Zhou Sa;Chen Qiuhong;Li Xinyue;Li Yingzhang
微管是一种高度动态的结构，在植物发育以及对环境信号和胁迫条件的响应中发挥着至关重要的作用。MT相关蛋白(MAP)在调节MT阵列的组织结构中起着重要作用。MAP65是一个植物MT结合蛋白家族。在这里，我们确定了MAP65-1在盐胁迫反应中的作用。MAP65-1不仅参与调节盐胁迫反应中的解聚，还参与随后的皮质MT的重组。此外，皮层MT的解聚影响了幼苗在盐胁迫下的存活，Map65-1突变体增强了对盐的敏感性。MAP65-1与丝裂原活化蛋白激酶(MPK)3和6相互作用，但只有pk6突变体对盐胁迫表现出超敏反应，MPK6参与调节盐胁迫诱导的皮质MTS解聚。因此，MAP65-1在对盐胁迫的反应中起着关键作用，是调节皮层MTS快速解聚和重组所必需的。MAP65-1与MPK6相互作用，而不是与MPK3相互作用，影响MT的动态不稳定性，这是植物耐盐性的关键。
Microtubules (MTs) are highly dynamical structures that play crucial roles in plant development and in response to environmental signals and stress conditions. MT-associated proteins (MAPs) play important roles in regulating the organization of MT arrays. MAP65 is a family of plant MT-bundling proteins. Here, we determined the role of MAP65-1 in the response to salt stress. MAP65-1 is involved not only in regulating the depolymerization, but also in the following reorganization of cortical MTs in salt stress responses. In addition, the depolymerization of the cortical MTs affected the survival of seedlings during salt stress, andmap65-1mutants had enhanced salt hypersensitivity levels. MAP65-1 interacted with mitogen-activated protein kinase (MPK) 3 and 6; however, only thempk6mutant exhibited hypersensitivity to salt stress, and MPK6 was involved in regulating the salt stress-induced depolymerization of cortical MTs. Thus, MAP65-1 plays a critical role in the response to salt stress and is required for regulating the rapid depolymerization and reorganization of cortical MTs. MAP65-1 interacts with MPK6, not MPK3, affecting the MT’s dynamic instability which is critical for plant salt-stress tolerance.