Efficient production of reuterin from glycerol by magnetically immobilized Lactobacillus reuteri
Efficient production of reuterin from glycerol by magnetically immobilized Lactobacillus reuteri
复制标题
磁固定罗伊氏乳杆菌从甘油高效生产罗伊氏菌素
DOI：
10.1007/s00253-015-6530-4
复制
发表时间：
2015-06-01
影响因子：
5
通讯作者：
Yu, Bo
中科院分区：
文献类型：
--
作者：
Liu, Feixia;Yu, Bo
Reurin是一种含有3-羟基丙醛(3-HPA)的单体、水合单体和环状二聚体的化合物。由于其抗菌性能，它被广泛用作食品防腐剂，3-HPA也是几种工业化学品的重要前体。在这项研究中，我们首次开发了一种利用磁性细胞捕获的高效固定化工艺，用于路氏乳杆菌从甘油中生产芦丁。采用统计学方法对细胞生长条件进行优化。以K-卡拉胶为固定化载体，以Fe3O4为磁性纳米粒子，获得了较高的转化率。此外，添加0.02g/L维生素B12、10 mmol/L镁(2+)、葡萄糖与甘油的摩尔比为0.15时，转化率显著提高。在最佳反应条件下，235.9 mmo1/L起始甘油在1.5h内合成了3-HPA，摩尔产率为94.4%，产率为15.4mmo1/(g干胞重∙∙h)，是迄今为止的最高值。因此，本研究为提高生物催化剂在生物转化中的效率提供了一条很有前途的途径。
Reuterin is a compound that contains the monomeric, hydrated monomeric, and cyclic dimeric forms of 3-hydroxypropionaldehyde (3-HPA). It is widely used as a food preservative due to its antimicrobial properties, and 3-HPA is also an important precursor for several industrial chemicals. In this study, we first developed an efficient immobilization process involving magnetic cell entrapment for production of reuterin from glycerol by Lactobacillus reuteri. The cell growth condition was optimized by statistical approaches. High conversion efficiency was achieved using k-carrageenan as the immobilization support and Fe3O4 as magnetic nanoparticles. Furthermore, addition of 0.02 g/L vitamin B12, 10 mmol/L Mg(2+), and glucose in a molar ratio of 0.15 to glycerol significantly increased the conversion rate. Under optimal conditions, 235.9 mmol/L 3-HPA was produced from 250 mmol/L initial glycerol in 1.5 h, with a molar yield of 94.4 % and a productivity of 15.4 mmol/(g dry cell weight∙L∙h), which were the highest values to date. Thus, this study demonstrated a promising process for the improvement of biocatalyst efficiency in biotransformation.