The Fc receptors of primary and cultured phagocytic cells studied with homogeneous antibodies.
The Fc receptors of primary and cultured phagocytic cells studied with homogeneous antibodies.
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使用均质抗体研究原代和培养的吞噬细胞的 Fc 受体。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
Matthew D. Scharff
中科院分区：
文献类型：
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作者：
Betty Diamond;Barry R. Bloom;Matthew D. Scharff
小鼠脾细胞-骨髓瘤杂交瘤产生分泌SRBC的IgG 2a和IgG 2b亚类的单克隆抗体。这些同质抗体用于检查驻留和巯基乙酸盐诱导的巨噬细胞和两种连续巨噬细胞样细胞系（J774.2和FC-1）上小鼠IgG 2a和IgG 2b的Fc受体。通过交叉抑制实验，我们已经表明，所有这四种细胞类型有不同的受体聚集的IgG 2a和IgG 2b。两种受体均结合与抗原复合的异质性兔抗体。大量的可溶性蛋白质不抑制抗原抗体复合物与任一受体的结合。用胰蛋白酶或细胞松弛素处理所有四种细胞类型可抑制IgG 2a包被的SRBC的玫瑰花结，但IgG 2b包被的SRBC的玫瑰花结不受抑制，并且在4°C下降低。IgG 2a和IgG 2b受体都将介导所有四种细胞类型中的吞噬作用。获得FC-1细胞系的突变体，其可以结合IgG 2a和IgG 2b抗体-抗原复合物，但仅通过IgG 2b受体吞噬。我们从这些研究中得出结论，抗原与小鼠IgG 2a和IgG 2b抗体之间天然存在的复合物与小鼠巨噬细胞结合，并通过功能上可区分的Fc受体被吞噬。使用均质单克隆抗体有利于此类研究，并避免了使用人工聚集的免疫球蛋白或异质性抗体时出现的一些模糊性。
Mouse spleen cell-myeloma hybrids were produced that secreted monoclonal antibodies to SRBC of the IgG2a and IgG2b subclass. These homogeneous antibodies were used to examine the Fc receptors for mouse IgG2a and IgG2b on resident and thioglycollate-induced macrophages and on two continuous macrophage-like cell lines (J774.2 and FC-1). By cross-inhibition experiments, we have shown that all four of these cell types have separate receptors for aggregated IgG2a and IgG2b. Both receptors bind heterogeneous rabbit antibody complexed to antigen. Large amounts of soluble proteins do not inhibit the binding of antigen-antibody complexes to either receptor. The rosetting of IgG2a-coated SRBC, but not of IgG2b-coated SRBC, is inhibited by treating all four cell types with trypsin or cytochalasin and is decreased at 4°C. Both the IgG2a and IgG2b receptors will mediate phagocytosis in all four cell types. Mutants of the FC-1 cell line were obtained that can bind both IgG2a and IgG2b antibody-antigen complexes but phagocytize only through the IgG2b receptor. We conclude from these studies that naturally occurring complexes between antigen and mouse IgG2a and IgG2b antibodies are bound to mouse macrophages and phagocytized through functionally distinguishable Fc receptors. The use of homogeneous monoclonal antibodies facilitates such studies and avoids some of the ambiguities that arise when artificially aggregated immunoglobulins or heterogeneous antibodies are used.