Nectarine promotes longevity in Drosophila melanogaster
Nectarine promotes longevity in Drosophila melanogaster
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DOI：
10.1016/j.freeradbiomed.2011.03.011
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发表时间：
2011-06-01
影响因子：
7.4
通讯作者：
Zou, Sige
中科院分区：
文献类型：
--
作者：
Boyd, Olga;Weng, Peter;Zou, Sige
含有高抗氧化能力和其他生物活性的水果是促进长寿和健康的理想选择。然而，很少有人知道水果可以提高动物的生存和健康寿命，更不用说潜在的机制了。在这里，我们调查油桃，一个全球消费的水果，对果蝇寿命和健康寿命的影响。野生型苍蝇喂食标准，饮食限制（DR），或高脂肪饮食补充0-4%油桃提取物。我们测量了这些苍蝇的寿命、食物摄入量、运动活动、繁殖力、基因表达变化以及由4-羟基壬烯醛蛋白加合物水平指示的氧化损伤。我们还测量了sod 1突变果蝇在标准饮食中添加0-4%油桃后的寿命、自发活动和氧化损伤。补充4%的油桃延长寿命，增加繁殖力，并降低一些代谢基因的表达，包括关键的代谢基因，PEPCK，和氧化应激反应基因，包括过氧化物酶，在雌性野生型苍蝇喂养的标准，DR，或高脂肪饮食。油桃降低了高脂饮食喂养的野生型雌性的氧化损伤。此外，油桃提高了雌性sod 1突变果蝇的存活率，并减少了氧化损伤。总之，这些发现表明，油桃部分通过调节葡萄糖代谢和减少氧化损伤来促进长寿和健康。爱思唯尔公司出版
Fruits containing high antioxidant capacities and other bioactivities are ideal for promoting longevity and health span. However, few fruits are known to improve the survival and health span in animals, let alone the underlying mechanisms. Here we investigate the effects of nectarine, a globally consumed fruit, on life span and health span in Drosophila melanogaster. Wild-type flies were fed standard, dietary restriction (DR), or high-fat diet supplemented with 0-4% nectarine extract. We measured life span, food intake, locomotor activity, fecundity, gene expression changes, and oxidative damage indicated by the level of 4-hydroxynonenal-protein adduct in these flies. We also measured life span, locomotor activity, and oxidative damage in sod1 mutant flies on the standard diet supplemented with 0-4% nectarine. Supplementation with 4% nectarine extended life span, increased fecundity, and decreased expression of some metabolic genes, including a key gluconeogenesis gene, PEPCK, and oxidative stress-response genes, including peroxiredoxins, in female wild-type flies fed the standard, DR, or high-fat diet. Nectarine reduced oxidative damage in wildtype females fed the high-fat diet. Moreover, nectarine improved the survival of and reduced oxidative damage in female sod1 mutant flies. Together, these findings suggest that nectarine promotes longevity and health span partly by modulating glucose metabolism and reducing oxidative damage. Published by Elsevier Inc.