The optx2 homeobox gene is expressed in early precursors of the eye and activates retina-specific genes.
The optx2 homeobox gene is expressed in early precursors of the eye and activates retina-specific genes.
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optx2 同源盒基因在眼睛的早期前体中表达并激活视网膜特异性基因。
DOI：
10.1073/pnas.95.18.10643
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发表时间：
1998
影响因子：
11.1
通讯作者：
Sundin,OH
中科院分区：
文献类型：
--
作者：
Toy,J;Yang,JM;Leppert,GS;Sundin,OH
脊椎动物眼的发育始于原肠胚期，此时眼区获得了产生视网膜和晶状体的能力，Optx 2是眼细胞Six家族的同源异型盒基因，选择性地在早期眼区表达，随后在透镜基板和视泡中表达。视泡的远端和腹侧部分注定成为视网膜和视神经，而背侧部分最终失去其神经特性并激活黑色素的合成，形成视网膜色素上皮。Optx 2在未来的色素上皮中表达关闭，但仍在神经视网膜的增殖神经母细胞和分化细胞中表达。当将表达Optx2的质粒转染到胚胎或成熟的鸡色素上皮细胞中时，这些细胞采用神经元形态并表达发育中的神经视网膜和光感受器的特征性标记。对这些结果的一种解释是，Optx2作为视网膜前体的决定因素发挥作用，并且它诱导了色素上皮向视网膜神经元和光感受器的转分化。我们也有分离的doptix，这是一个果蝇基因，它是与Optx2和Six3最接近的昆虫同源物。Optixis在苍蝇头部和眼睛原基的早期发育过程中表达。
Vertebrate eye development begins at the gastrula stage, when a region known as the eye field acquires the capacity to generate retina and lens.Optx2, a homeobox gene of thesine oculis-Sixfamily, is selectively expressed in this early eye field and later in the lens placode and optic vesicle. The distal and ventral portion of the optic vesicle are fated to become the retina and optic nerve, whereas the dorsal portion eventually loses its neural characteristics and activates the synthesis of melanin, forming the retinal pigment epithelium.Optx2expression is turned off in the future pigment epithelium but remains expressed in the proliferating neuroblasts and differentiating cells of the neural retina. When anOptx2-expressing plasmid is transfected into embryonic or mature chicken pigment epithelial cells, these cells adopt a neuronal morphology and express markers characteristic of developing neural retina and photoreceptors. One explanation of these results is thatOptx2functions as a determinant of retinal precursors and that it has induced the transdifferentiation of pigment epithelium into retinal neurons and photoreceptors. We also have isolatedoptix, aDrosophilagene that is the closest insect homologue ofOptx2andSix3. Optixis expressed during early development of the fly head and eye primordia.