Dependence of Cardiac Trabeculation on Neuregulin Signaling and Blood Flow in Zebrafish
Dependence of Cardiac Trabeculation on Neuregulin Signaling and Blood Flow in Zebrafish
复制标题
DOI：
10.1002/dvdy.22526
复制
发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Yelon, Deborah
中科院分区：
文献类型：
--
作者：
Peshkovsky, Courtney;Totong, Ronald;Yelon, Deborah
发育中的心脏的成熟需要通过小梁形成过程对胚胎脑室的结构进行细化。当心室肌伸入腔内时形成小梁，从而增加肌肉质量并改变功能输出。人们对骨小梁形成的细胞基础及其基因调控知之甚少。在这里，我们建立了斑马鱼胚胎用于分析驱动小梁形成的机制。在斑马鱼中，我们可以在四个维度上跟踪小梁形成，并在形态上定义形成脑室小梁的起始、繁殖和网络细化的不同阶段。我们发现，NeuRegin/ErbB信号是心肌最初突入脑室腔所必需的。此外，我们还证明了最佳的脑室血流对于小梁形成的进展是重要的。因此，我们的结果表明，斑马鱼为研究骨小梁先天性缺陷的可能原因提供了一个有价值的模型。《发展动力学》240：446-456,2011年。(C)2011年Wiley-Liss，Inc.
Maturation of the developing heart requires the structural elaboration of the embryonic ventricle through the process of trabeculation. Trabeculae form as the ventricular myocardium protrudes into the lumen of the chamber, thereby increasing muscle mass and altering functional output. Little is understood about the cellular basis for trabeculation and its genetic regulation. Here, we establish the utility of the zebrafish embryo for the analysis of the mechanisms driving trabeculation. In zebrafish, we can follow trabeculation in four dimensions and define morphologically discrete stages for the initiation, propagation, and network elaboration that form the ventricular trabeculae. We find that Neuregulin/ErbB signaling is required for the initial protrusion of the myocardium into the ventricular lumen. Additionally, we demonstrate that optimal blood flow through the ventricle is important for the advancement of trabeculation. Thus, our results indicate that the zebrafish provides a valuable model for investigating possible causes of congenital defects in trabeculation. Developmental Dynamics 240:446-456, 2011. (C) 2011 Wiley-Liss, Inc.