GATA-4 is a novel transcription factor expressed in endocardium of the developing heart.
GATA-4 is a novel transcription factor expressed in endocardium of the developing heart.
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发表时间：
1993-07
期刊：
影响因子：
4.6
通讯作者：
C. Kelley;Heather Blumberg;Leonard I. Zon;Todd Evans
中科院分区：
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作者：
C. Kelley;Heather Blumberg;Leonard I. Zon;Todd Evans
我们分离并鉴定了一种新的转录因子的爪蟾cDNA克隆，该转录因子代表了发育中的心脏的早期标记。这些cdna编码一种蛋白质，我们称之为GATA-4；它包含高度保守的dna结合域，这是这个细胞型限制性转录激活因子家族的特征。对非洲爪蟾胚胎的全胚胎原位分析表明，在双侧祖细胞融合形成心管时，GATA-4基因在假定的心脏腹侧中胚层中转录。因此，GATA-4是目前已知的最早的心脏发生分子标记物。到30期，GATA-4 mRNA在发育中的心房和心室中表达；在第38期，横断面显示该基因在心内膜层活跃，但在心肌中不活跃。到第40阶段，在大血管中检测到GATA-4信息。在成年青蛙中，GATA-4基因在心脏和肠道中高度转录；在各种内胚层来源的组织和性腺中检测到较低水平的信息。胃的表达主要局限于上皮。GATA-4基因首先在11期被激活；mRNA最初存在于整个外植体边缘区，后来部分定位于腹侧边缘区。GATA-4 mRNA在早期胚胎植物区培养的内胚层外植体中也有高水平的表达。在中胚层诱导实验中，激活素或bFGF处理的动物帽不诱导GATA-4转录。GATA-4基因可能为研究心血管系统形成的诱导过程和心内膜谱系的确定提供一个新的早期标记。
We have isolated and characterized Xenopus cDNA clones for a new transcription factor that represents an early marker for the developing heart. The cDNAs encode a protein that we have designated GATA-4; it contains the highly conserved DNA-binding domain that characterizes this family of cell-type restricted transcriptional activators. Whole-embryo in situ analysis of Xenopus embryos demonstrates that the GATA-4 gene is transcribed in presumptive cardiac ventral mesoderm at the time that bilateral progenitors fuse and form the cardiac tube. GATA-4 is therefore the earliest molecular marker of cardiogenesis yet characterized. By stage 30, the GATA-4 mRNA is expressed in the developing atria and ventricles; at stage 38, cross-sections reveal that the gene is active in the endocardial layer, but not in myocardium. By stage 40, GATA-4 message is detected in the great vessels. In the adult frog, the GATA-4 gene is highly transcribed in heart and gut; lower levels of message are detected in various endoderm-derived tissues and gonads. Expression in the stomach is largely confined to the epithelium. The GATA-4 gene is first activated at stage 11; mRNA is initially present throughout the marginal zone of explants and later partially localized to the ventral marginal zone. GATA-4 mRNA is also detected at high levels in cultured endodermal explants derived from the vegetal region of early embryos. In mesoderm induction experiments, GATA-4 transcription is not induced in animal caps treated with activin or bFGF. The GATA-4 gene may provide a new early marker for studying the inductive processes that lead to the formation of the cardiovascular system and for the specification of the endocardial lineage.