Superoxide dismutase SodB is a protective antigen against Campylobacter jejuni colonisation in chickens.
Superoxide dismutase SodB is a protective antigen against Campylobacter jejuni colonisation in chickens.
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DOI：
10.1016/j.vaccine.2015.09.100
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发表时间：
2015-11-17
期刊：
影响因子：
5.5
通讯作者：
Stevens MP
中科院分区：
文献类型：
--
作者：
Chintoan-Uta C;Cassady-Cain RL;Al-Haideri H;Watson E;Kelly DJ;Smith DG;Sparks NH;Kaiser P;Stevens MP
我们将SoDB作为抗弯曲菌重组亚单位疫苗在鸡身上进行了试验。它诱导盲肠空肠弯曲菌在统计学上显著减少。抗原特异性体液反应与保护性无关。细菌表面未检测到SODB。保护可能不是严格依赖于抗体的。弯曲杆菌是发达国家食源性腹泻疾病的主要原因，食用或处理受污染的禽肉是感染源的主要来源。迫切需要在屠宰前控制肉鸡中的弯曲杆菌的战略，预计这将限制人类弯曲杆菌病的发生率。为此，我们研制了一种基于空肠弯曲菌M1超氧化物歧化酶(SoDB)蛋白的重组亚单位疫苗，并在白色来航鸡中进行了试验。分别于孵化当天和14日龄后接种与谷胱甘肽S转移酶融合的SoDB或单独接种纯化的GST。在28日龄时用空肠弯曲菌M1攻击雏鸡，每周测定一次盲肠弯曲菌计数。在三项独立试验中，与年龄匹配的GST疫苗接种对照组相比，接种疫苗的禽类盲肠弯曲杆菌数量在统计上显著减少了1log10。在所有接种疫苗的禽类中都检测到了显著的抗原特异性血清IgY的诱导，但SoDB特异性IgY的大小和时间与空肠弯曲菌数量较少无关。来自SoDB疫苗的鸡的抗体在周质中检测到该蛋白，而不是膜部分或细菌表面，这表明观察到的保护可能不是严格的抗体介导的。SODB可能是控制肉鸡空肠弯曲菌感染疫苗的有效成分，但相对于肉鸡的寿命，观察到的保护较晚，需要进一步研究以加强保护的大小和时机。
We tested SodB in chickens as an anti-Campylobacter recombinant subunit vaccine. It induced a statistically significant reduction in caecal C. jejuni. Antigen-specific humoral responses did not correlate with protection. SodB was not detected on the bacterial surface. Protection may not be strictly antibody-dependent. Campylobacter is the leading cause of foodborne diarrhoeal illness in the developed world and consumption or handling of contaminated poultry meat is the principal source of infection. Strategies to control Campylobacter in broilers prior to slaughter are urgently required and are predicted to limit the incidence of human campylobacteriosis. Towards this aim, a purified recombinant subunit vaccine based on the superoxide dismutase (SodB) protein of C. jejuni M1 was developed and tested in White Leghorn birds. Birds were vaccinated on the day of hatch and 14 days later with SodB fused to glutathione S-transferase (GST) or purified GST alone. Birds were challenged with C. jejuni M1 at 28 days of age and caecal Campylobacter counts determined at weekly intervals. Across three independent trials, the vaccine induced a statistically significant 1 log10 reduction in caecal Campylobacter numbers in vaccinated birds compared to age-matched GST-vaccinated controls. Significant induction of antigen-specific serum IgY was detected in all vaccinated birds, however the magnitude and timing of SodB-specific IgY did not correlate with lower numbers of C. jejuni. Antibodies from SodB-vaccinated chickens detected the protein in the periplasm and not membrane fractions or on the bacterial surface, suggesting that the protection observed may not be strictly antibody-mediated. SodB may be useful as a constituent of vaccines for control of C. jejuni infection in broiler birds, however modest protection was observed late relative to the life of broiler birds and further studies are required to potentiate the magnitude and timing of protection.