The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice
The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice
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DOI：
10.1111/nph.15448
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发表时间：
2019-01-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Zhiguo
中科院分区：
文献类型：
--
作者：
Cui, Xuean;Wang, Yanwei;Zhang, Zhiguo
低温胁迫阻碍植物生长和叶绿体发育，并限制品种的地理范围。在水稻中，粳稻品种比籼稻品种具有更强的耐冷性，但耐冷性的分子机制尚不清楚。在这里，我们报告了一个RNA结合蛋白，DUA 1，克隆从籼稻品种Dular，这表现出在低温条件下发展的早期阶段叶绿体发育的缺陷。DUA 1与pentatricopeptide repeat家族具有高度的序列同源性，并在低温条件下在质体RNA编辑中发挥作用。我们的数据表明，DUA 1可以结合到质体编码的rps 8 -182转录本和DUA 1活性的破坏损害编辑。RNA编辑辅因子WSP 1是DUA 1的伴侣，也参与低温下叶绿体的发育。Western blot分析表明，WSP 1增强了DUA 1在低温下的稳定性。水稻核心种质DUA 1序列分析表明，DUA 1的3个主要单倍型和1个单倍型在低温条件下的叶绿素含量存在显著差异。DUA 1的变异可能在水稻适应不同生长区域中起重要作用。
Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA-binding protein, DUA1, cloned from the indica cultivar Dular, which exhibits a deficiency in chloroplast development at an early stage of development under low-temperature conditions. DUA1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low-temperature conditions. Our data suggest that DUA1 can bind to the plastid-encoded rps8-182 transcript and disruption of DUA1 activity impairs editing. The RNA editing cofactor WSP1, a partner of DUA1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures. DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low-temperature conditions. Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.