Bacterial anoxygenic photosynthesis on plant leaf surfaces
Bacterial anoxygenic photosynthesis on plant leaf surfaces
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DOI：
10.1111/j.1758-2229.2011.00323.x
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Beja, Oded
中科院分区：
文献类型：
--
作者：
Atamna-Ismaeel, Nof;Finkel, Omri;Beja, Oded
植物的气生表面，即叶圈，被许多细菌定殖，这些细菌表现出不同的代谢特性，使它们能够在这个特定的栖息地中生存。最近，我们报道了存在于叶片表面的微生物视紫红质窝藏细菌。在这里，我们报告了额外的细菌种群的存在，能够收集光作为补充其代谢需求的一种手段。对六个叶际宏基因组的分析揭示了存在不同的缺氧光养细菌群落，包括先前报道的甲基杆菌，以及其他已知和未知的光养生物。通过红外荧光显微镜原位观察，也证实了不产氧光合细菌的存在。显微计数与基于宏基因组分析的估计值相关，证实了这些微生物在叶际中的存在和高丰度。我们的数据表明，叶圈包含一个共生的光合物种，包括一些尚未描述的细菌分支，似乎是叶圈独特的多样性组合。
The aerial surface of plants, the phyllosphere, is colonized by numerous bacteria displaying diverse metabolic properties that enable their survival in this specific habitat. Recently, we reported on the presence of microbial rhodopsin harbouring bacteria on the top of leaf surfaces. Here, we report on the presence of additional bacterial populations capable of harvesting light as a means of supplementing their metabolic requirements. An analysis of six phyllosphere metagenomes revealed the presence of a diverse community of anoxygenic phototrophic bacteria, including the previously reported methylobacteria, as well as other known and unknown phototrophs. The presence of anoxygenic phototrophic bacteria was also confirmed in situ by infrared epifluorescence microscopy. The microscopic enumeration correlated with estimates based on metagenomic analyses, confirming both the presence and high abundance of these microorganisms in the phyllosphere. Our data suggest that the phyllosphere contains a phylogenetically diverse assemblage of phototrophic species, including some yet undescribed bacterial clades that appear to be phyllosphere-unique.