Fluorescence techniques in biotechnology
Fluorescence techniques in biotechnology
复制标题
生物技术中的荧光技术
DOI：
10.1016/0167-7799(85)90025-3
复制
发表时间：
1985
影响因子：
17.3
通讯作者：
S. Schoberth
中科院分区：
文献类型：
--
作者：
H. Schneckenburger;B. Reuter;S. Schoberth
荧光技术的高特异性和高灵敏度使其成为医学和生物技术中重要的分析工具。除了监测和定量检测生物分子外，这些方法还可用于控制细菌活动或测量细胞或组织的生理状态。详细介绍了生物工程中的三个重要课题--免疫分析、光合作用和发酵。许多有机分子，特别是具有双键的芳香烃，在光谱的可见光或近紫外区的光激发下发出特征荧光。荧光产生于分子的最低激发态到基态的光学跃迁(图1)，可用于分析生物分子、色素、辅酶或某些
The high specificity and sensitivity of fluorescence techniques have made them important analytical tools in medicine and biotechnology. Besides monitoring and quantitative detection of biomolecules these methods can be used for controlling bacterial activities or for measuring physiological states of cells or tissues. Three topics of importance in biotechnology-immunoassays, photosynthesis and fermentation-are treated in detail.Many organic molecules, particularly aromatic hydrocarbons with double bonds, emit characteristic fluorescence after excitation by light from the visible or near UV region of the spectrum. The fluorescence arises from an optical transition of the lowest excited state to the ground state of the molecules (Fig. 1) and can be used for analysing biomolecules, pigments, coenzymes or certain