Endothelial-like cells derived from human CD14 positive monocytes
Endothelial-like cells derived from human CD14 positive monocytes
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DOI：
10.1046/j.1432-0436.2000.6550287.x
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发表时间：
2000-05-01
期刊：
影响因子：
2.9
通讯作者：
Havemann, K
中科院分区：
文献类型：
--
作者：
Pujol, BF;Lucibello, FC;Havemann, K
在目前的研究中，我们表明，内皮样细胞（ELC）可以开发从人CD 14阳性单核细胞（CD 14细胞）的血管生成生长因子的存在下。CD 14细胞在培养24小时内变得松散粘附，随后经历了一个独特的形态学转化过程，向具有偏心核的尾状或椭圆形细胞转化。培养1周后，细胞显示出内皮细胞标志物的明确表达，包括血管性血友病因子（vWF）、CD 144（VE-钙粘蛋白）、CD 105（内皮糖蛋白）、乙酰化低密度脂蛋白（AC-LDL）受体、CD 36（血小板反应蛋白受体）、FLT-1（血管内皮细胞生长因子（VEGF）受体-1）和较弱程度的KDR（VEGF受体-2）。此外，在这些细胞中，在不同的储存阶段类似于韦伯-帕拉德体的结构通过电子显微镜进行鉴定，并且在三维纤维蛋白凝胶上培养时，细胞构建网络状结构。此外，细胞增殖和vWF表达被VEGF刺激，并且内皮细胞粘附分子CD 54（ICAM-1）和CD 106（VCAM-1）被肿瘤坏死因子-α（TNF-α）瞬时诱导。与此相反，树突状细胞标志物CD 1a和CD 83没有表达到任何显着的程度。CD 68、CD 80（B7-1）、CD 86（B7-2）、HLA-DR和CD 36的表达也提示ELC可能与巨噬细胞、窦衬里或微血管内皮细胞有关。两者合计，我们的观察表明，ELC可以从单核细胞谱系的细胞分化，这表明单核细胞/巨噬细胞和内皮细胞系统之间的关系比以前假设的更密切。
In the present study, we show that endothelial-like cells (ELCs) can develop from human CD14-positive mononuclear cells (CD14 cells) in the presence of angiogenic growth factors. The CD14 cells became loosely adherent within 24 h of culture and subsequently underwent a distinct process of morphological transformation to caudated or oval cells with eccentric nuclei. After 1 week in culture the cells showed a clear expression of endothelial cell markers, including von Willebrand factor (vWF), CD144 (VE-cadherin), CD105 (endoglin), acetylated low-density lipoprotein (AC-LDL)-receptor, CD36 (thrombospondin receptor), FLT-1, which is vascular endothelial cell growth factor (VEGF) receptor-1, and, to a weaker extent, KDR (VEGF receptor-2). Furthermore, in these cells structures resembling Weibel-Palade bodies at different storage stages were identified by electron microscopy, and upon culturing on three-dimensional fibrin gels the cells build network-like structures. In addition, cell proliferation and vWF expression was stimulated by VEGF, and the endothelial cell adhesion molecules CD54 (ICAM-1), and CD106 (VCAM-1) became transiently inducible by tumor necrosis factor-alpha (TNF-alpha). In contrast, the dendritic markers CD1a, and CD83 were not expressed to any significant extent. The expression of CD68, CD80 (B7-1), CD86 (B7-2), HLA-DR and CD36 may also suggest that ELCs might be related to macrophages, sinus lining or microvascular endothelial cells. Taken together, our observations indicate that ELCs can differentiate from cells of the monocytic lineage, suggesting a closer relationship between the monocyte/macrophage- and the endothelial cell systems than previously supposed.