SENDAI VIRUS CONTAINS OVERLAPPING GENES EXPRESSED FROM A SINGLE MESSENGER-RNA
SENDAI VIRUS CONTAINS OVERLAPPING GENES EXPRESSED FROM A SINGLE MESSENGER-RNA
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DOI：
10.1016/0092-8674(83)90115-0
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
KOLAKOFSKY, D
中科院分区：
文献类型：
--
作者：
GIORGI, C;BLUMBERG, BM;KOLAKOFSKY, D
用克隆的DNA将编码仙台病毒P蛋白和C蛋白的信使核糖核酸定位在病毒基因组上，并对DNA的相关区域进行了测序。核苷酸序列显示两个重叠的开放阅读框，可编码568和204个氨基酸的蛋白质。引物延伸和S1核酸酶作图研究仅从该区域检测到一个1.894 kb碱基的mRNA.使用限制片段的混合抑制翻译研究证实了这些基因的重叠性质。仙台病毒3个顺反子开头的序列同源性表明，这些基因可能来自共同的祖先，可能是流感样病毒基因。
The mRNA coding for the Sendai virus P and C proteins was located on the viral genome using cloned DNA and the relevant regions of the DNA were sequenced. The nucleotide sequence revealed 2 overlapping open reading frames that could code for proteins of 568 and 204 amino acids. Primer extension and S1 nuclease mapping studies detected only a single 1.894 kilobase mRNA from this region. Hybrid arrest of translation studies using restriction fragments verified the overlapping nature of these genes. Sequence homologies at the beginning of 3 Sendai virus cistrons suggest that these genes may have arisen by duplication from a common ancestor, possibly an influenza-like virus gene.