Optimizing cell viability in droplet-based cell deposition.
Optimizing cell viability in droplet-based cell deposition.
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DOI：
10.1038/srep11304
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发表时间：
2015-06-11
影响因子：
4.6
通讯作者：
Karperien M
中科院分区：
文献类型：
--
作者：
Hendriks J;Willem Visser C;Henke S;Leijten J;Saris DB;Sun C;Lohse D;Karperien M
生物制造通常涉及使用液滴将细胞输送到印刷结构。然而，撞击后细胞的活性缺乏控制和了解，阻碍了细胞喷洒、喷墨生物打印和激光辅助细胞转移等应用。在这里，我们提出了一个分析模型，描述了撞击后细胞活力作为细胞周围液滴特征的函数。该模型连接(1)细胞存活率作为细胞膜伸长的函数，(2)膜伸长作为含有细胞的液滴大小和速度的函数，以及(3)底物性质。细胞喷雾是一种用于治疗烧伤创面的生物制造方法，通过细胞喷雾的细胞活性测量对该模型进行了验证。这些结果允许合理地优化任何基于液滴的细胞沉积技术，并包括在细胞喷涂中提高细胞活力的实用建议。
Biofabrication commonly involves the use of liquid droplets to transport cells to the printed structure. However, the viability of the cells after impact is poorly controlled and understood, hampering applications including cell spraying, inkjet bioprinting, and laser-assisted cell transfer. Here, we present an analytical model describing the cell viability after impact as a function of the cell-surrounding droplet characteristics. The model connects (1) the cell survival as a function of cell membrane elongation, (2) the membrane elongation as a function of the cell-containing droplet size and velocity, and (3) the substrate properties. The model is validated by cell viability measurements in cell spraying, which is a method for biofabrication and used for the treatment of burn wounds. The results allow for rational optimization of any droplet-based cell deposition technology, and we include practical suggestions to improve the cell viability in cell spraying.