Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation.
Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation.
复制标题
DOI：
10.1016/j.pacs.2017.03.003
复制
发表时间：
2017-03
期刊：
影响因子：
7.9
通讯作者：
Kolios MC
中科院分区：
文献类型：
--
作者：
Hysi E;Wirtzfeld LA;May JP;Undzys E;Li SD;Kolios MC
光声射频信号的频率分析和血氧饱和度估计被用来监测一种新型的热敏性脂质体治疗的体内反应。该脂质体包裹阿霉素(HAT-DOX)，当肿瘤暴露在43℃的轻度高温下时，迅速释放它(<；20来自S)。对EMT-6乳腺癌肿瘤进行光声成像(VevoLAZR，750/850 nm，40 MHz)，在治疗前、治疗后30min和治疗后7d(2/5/24小时时间点)进行扫描。HAT-DOX治疗应答者在治疗后2小时血氧饱和度平均下降22%，归一化PA频谱斜率下降(750 nm处45%，850 nm处73%)。光谱斜率参数与治疗引起的出血有关，后者通过间质红细胞渗漏增加了光吸收体的有效尺寸。结合频率分析和血氧饱和度，通过探测治疗引起的血管结构变化，估计区分治疗应答者和无应答者/对照动物。
Frequency analysis of the photoacoustic radiofrequency signals and oxygen saturation estimates were used to monitor the in-vivo response of a novel, thermosensitive liposome treatment. The liposome encapsulated doxorubicin (HaT-DOX) releasing it rapidly (<20 s) when the tumor was exposed to mild hyperthermia (43 °C). Photoacoustic imaging (VevoLAZR, 750/850 nm, 40 MHz) of EMT-6 breast cancer tumors was performed 30 min pre- and post-treatment and up to 7 days post-treatment (at 2/5/24 h timepoints). HaT-DOX-treatment responders exhibited on average a 22% drop in oxygen saturation 2 h post-treatment and a decrease (45% at 750 nm and 73% at 850 nm) in the slope of the normalized PA frequency spectra. The spectral slope parameter correlated with treatment-induced hemorrhaging which increased the optical absorber effective size via interstitial red blood cell leakage. Combining frequency analysis and oxygen saturation estimates differentiated treatment responders from non-responders/control animals by probing the treatment-induced structural changes of blood vessel.