Population Response Propagation to Extrastriate Areas Evoked by Intracortical Electrical Stimulation in V1.
Population Response Propagation to Extrastriate Areas Evoked by Intracortical Electrical Stimulation in V1.
复制标题
DOI：
10.3389/fncir.2016.00006
复制
发表时间：
2016
影响因子：
3.5
通讯作者：
Yagi T
中科院分区：
文献类型：
--
作者：
Fehérvári TD;Yagi T
小鼠视觉系统在V1周围有多个纹外区域，每个区域都有不同的视野和独特的功能和连接轮廓，它们被认为形成了两个平行的处理流，类似于灵长类动物的腹流和背流。同时，鼠标可视区域具有高度的互连性，特别是V1将输入发送到所有更高的可视区域。对这些直接联系的研究可以加深我们对早期哺乳动物皮质中视觉信号的皮质处理的理解。关于这些连接的解剖结构已经发表了几项研究，但体内电生理特征和对多个纹状体外区域传递的比较尚未见报道。我们在成年C57BL/6小鼠乌拉坦麻醉下，采用皮层内电刺激结合RH1691 VSD成像的方法，分析了V1到皮层浅层纹外区的区域间传导。我们发现了7个纹外反应部位(5个外侧，2个内侧)，其空间模式类似于小鼠视皮层的区域图，并通过改变刺激V1的位置，证明了在LM和AL中的诱发反应与解剖学和活体研究中已知的这些区域的视觉映射一致。这两个站点被认为是处理流的网关，与其他条带外响应站点相比，它们具有更短的延迟和更快的传输速度。反应部位之间的短潜伏期差异，以及TTX注入Lm减少但没有消除其他纹外反应，表明诱发的皮质活动至少部分地从V1直接传递到纹外区域。本研究在小鼠大脑皮质内电刺激的基础上，分析了V1区向多个纹外区的区域传递。
The mouse visual system has multiple extrastriate areas surrounding V1 each with a distinct representation of the visual field and unique functional and connectivity profiles, which are believed to form two parallel processing streams, similar to the ventral and dorsal streams in primates. At the same time, mouse visual areas have a high degree of interconnectivity, in particular V1 sends input to all higher visual areas. The study of these direct connections can further our understanding of the cortical processing of visual signals in the early mammalian cortex. Several studies have been published about the anatomy of these connections, but an in vivo electrophysiological characterization and comparison of the transmission to multiple extrastriate areas has not yet been reported. We used intracortical electrical stimulation combined with RH1691 VSD imaging in adult C57BL/6 mice in urethane anesthesia to analyze interareal transmission from V1 to extrastriate areas in superficial cortical layers. We found seven extrastriate response sites (five lateral, two medial) in a spatial pattern similar to area maps of the mouse visual cortex and, by shifting the location of V1 stimulation, demonstrated that the evoked responses in LM and AL were in accordance with the visuotopic mappings of these areas known from anatomy and in vivo studies. These two sites, considered to be gateways to their processing streams, had shorter latencies and faster transmission speeds than other extrastriate response sites. Short latency differences between response sites, and that TTX injection into LM reduced but did not eliminate other extrastriate responses indicated that the evoked cortical activity was, at least partially, transmitted directly from V1 to extrastriate areas. This study reports on analysis of interareal transmission from V1 to multiple extrastriate areas in mouse using intracortical electrical stimulation in vivo.