Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
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单一苯并[a]芘二醇环氧化物-脱氧鸟苷加合物对体外DNA聚合酶作用的影响。
DOI：
10.3892/ijo.13.2.269
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发表时间：
1998
影响因子：
5.2
通讯作者：
A. Dipple
中科院分区：
文献类型：
--
作者：
L. J. Lipinski;H. Ross;B. Zajc;J. M. Sayer;D. Jerina;A. Dipple
Sequenase 2.0和Klenow fragment都不能使用8个模板（16-mers）来延伸12-mer引物，这些模板是通过将四个异构体苯并[a]芘二醇环氧-脱氧鸟苷加合物分别放置在两个不同序列背景的3'端第13个核苷酸上而得到的。使用11-mer引物启动并没有克服加合物诱导的引物延伸障碍，除了Klenow片段和两个序列上下文之一，这表明引物延伸依赖于聚合酶和序列上下文。在这种情况下，嘌呤核苷三磷酸（dATP>dGTP）与四种加合物分别结合。
Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.