Histomorphometric studies show that bone formation and bone mineral apposition rates are greater in C3H/HeJ (high-density) than C57BL/6J (low-density) mice during growth.
Histomorphometric studies show that bone formation and bone mineral apposition rates are greater in C3H/HeJ (high-density) than C57BL/6J (low-density) mice during growth.
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组织形态计量学研究表明，在生长过程中，C3H/HeJ（高密度）小鼠的骨形成和骨矿物质沉积率高于 C57BL/6J（低密度）小鼠。
DOI：
10.1016/s8756-3282(99)00184-2
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发表时间：
1999
期刊：
影响因子：
4.1
通讯作者：
Wergedal,JE
中科院分区：
文献类型：
--
作者：
Sheng,MH;Baylink,DJ;Beamer,WG;Donahue,LR;Rosen,CJ;Lau,KH;Wergedal,JE
选择股骨骨密度相差 50% 的高密度 C3H/HeJ (C3H) 和低密度 C57BL/6J (B6) 小鼠作为模型，研究控制峰值骨密度的机制并绘制峰值骨密度基因图谱。目前的纵向研究是为了进一步确定这两种近交系小鼠的骨生物学表型。为了评估 6 周龄和 26 周龄 C3H 和 B6 小鼠骨形成参数的表型差异，从接受两次四环素注射的小鼠中制备胫骨和股骨骨干的未脱钙地面切片。组织形态计量学分析显示，在所有时间点，C3H 小鼠股骨和胫骨的皮质骨面积均显着大于 B6 小鼠（16%–56%，p < 0.001）。皮质骨面积的这种差异是由于 C3H 小鼠的髓质面积明显小于 B6 小鼠的髓质面积。年轻 C3H 小鼠（6-12 周龄）股骨和胫骨骨内膜的骨形成率 (BFR) 显着高于 B6 小鼠（28%–117%，p < 0.001）。 C3H 小鼠较高的骨形成与较高的骨矿物质沉积率相关（25%–94%，p < 0.001），并且与通过四环素标记长度测量的较高的形成表面长度值无关。在股骨和胫骨的骨膜中观察到类似的矿物沉积和骨形成率的种间差异。总之，高密度 C3H 小鼠与低密度 B6 小鼠相比，骨面积更大，部分原因是 C3H 小鼠生长过程中骨膜和骨内膜骨形成率更高。由于C3H和B6小鼠饲养在相同的环境条件（饮食、光照等）下，观察到的骨参数的种间差异是遗传因素作用的结果。因此，这两种近交系小鼠适合作为模型来识别导致高骨形成率的遗传因素。
High-density C3H/HeJ (C3H) and low-density C57BL/6J (B6) mice, with femoral bone density differing by 50%, were chosen as a model to investigate the mechanisms controlling peak bone density and to map peak bone density genes. The present longitudinal study was undertaken to further establish the bone biologic phenotypes of these two inbred strains of mice. To evaluate phenotypic differences in bone formation parameters in C3H and B6 mice between the ages of 6 and 26 weeks, undecalcified ground sections from the diaphyses of the tibia and femur were prepared from mice receiving two injections of tetracycline. Histomorphometric analyses revealed that the cortical bone area was significantly greater (16%–56%, p < 0.001) in both the femur and tibia of the C3H mice than in the B6 mice at all timepoints. This difference in cortical bone area was due to significantly smaller medullary areas in the C3H mice than in the B6 mice. The bone formation rates (BFR) at the endosteum in both the femur and tibia were significantly greater (28%–117%, p < 0.001) in the young C3H mice (6–12 weeks old) than in B6 mice. The higher bone formation in C3H mice was associated with higher values of the bone mineral apposition rate (25%–94%, p < 0.001), and was not associated with higher values of the forming surface length as measured by tetracycline label length. Similar interstrain differences in mineral apposition and bone formation rates were observed in the periosteum of the femur and tibia. In conclusion, the greater bone area in the high-density C3H mice vs. the low-density B6 mice was, in part, due to the greater periosteal and endosteal bone formation rates during growth in the C3H mice. Because the C3H and B6 mice were maintained under identical environmental conditions (diet, lighting, etc.), the observed interstrain differences in bone parameters were the result of the action of genetic factors. Consequently, these two inbred strains of mice are suitable as a model to identify genetic factors responsible for high bone formation rates.