Histologic and Immunohistochemical Evaluation of Infiltrating Inflammatory Cells in Kawasaki Disease Arteritis Lesions
Histologic and Immunohistochemical Evaluation of Infiltrating Inflammatory Cells in Kawasaki Disease Arteritis Lesions
复制标题
DOI：
10.1097/pai.0000000000000860
复制
发表时间：
2021-01-01
影响因子：
1.6
通讯作者：
Kanno, Hiroyuki
中科院分区：
文献类型：
--
作者：
Kobayashi, Mikiko;Matsumoto, Yuki;Kanno, Hiroyuki
川崎是一种病因不明的系统性血管炎，主要累及中小肌性动脉。KD血管炎病变的组织学研究已经证实了特征性T细胞浸润和丰富的CD 8（+）T细胞;然而，尚未确定细胞毒性淋巴细胞对KD血管炎病变的作用。在此，我们对KD血管炎病变中浸润的炎性细胞，特别是细胞毒性蛋白阳性细胞，如颗粒酶B+细胞和TIA-1（+）细胞进行了组织病理学和免疫化学检查。观察到3例急性期KD尸检标本，其中24例血管炎病变累及中型肌动脉，不包括肺动脉。通过苏木精和伊红染色评价血管炎病变中浸润的中性粒细胞，并通过免疫组织化学评价单核细胞/巨噬细胞和淋巴细胞。主要细胞为CD 163（+）单核/巨噬细胞和CD 3（+）T细胞。还观察到CD 8（+）T细胞、颗粒酶B+细胞和TIA-1（+）细胞，但CD 56（+）自然杀伤细胞很少。据我们所知，目前的研究是第一个组织病理学报告证实炎症细胞浸润细胞毒性蛋白在血管炎病变的KD患者。细胞毒性T细胞可能在KD患者血管炎病变的发展中发挥作用。
Kawasaki disease (KD) is a systemic vasculitis of unknown etiology which predominantly affects medium- and small-sized muscular arteries. Histopathologic studies of KD vasculitis lesions have demonstrated characteristic T cell infiltration and an abundance of CD8(+) T cells; however, the contribution of cytotoxic lymphocytes to KD vasculitis lesions has not been identified. Here, we histopathologically and immunohistochemically examined infiltrating inflammatory cells, particularly cytotoxic protein-positive cells, such as granzyme B+ cells and TIA-1(+) cells, in KD vasculitis lesions. Three autopsy specimens with acute-phase KD were observed and contained 24 vasculitis lesions affecting medium-sized muscular arteries, excluding pulmonary arteries. Infiltrating neutrophils in vasculitis lesions were evaluated by hematoxylin and eosin staining, and monocytes/macrophages and lymphocytes were evaluated by immunohistochemistry. The predominant cells were CD163(+) monocytes/macrophages and CD3(+) T cells. CD8(+) T cells, granzyme B+ cells, and TIA-1(+) cells were also observed, but CD56(+) natural killer cells were rare. To the best of our knowledge, the current study is the first histopathologic report confirming the infiltration of inflammatory cells with cytotoxic proteins in vasculitis lesions in patients with KD. Cytotoxic T cells may play a role in the development of vasculitis lesions in KD patients.