Molecular alterations in a new cell line (KU-Lu-MPPt3) established from a human lung adenocarcinoma with a micropapillary pattern
Molecular alterations in a new cell line (KU-Lu-MPPt3) established from a human lung adenocarcinoma with a micropapillary pattern
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从具有微毛细管模式的人肺腺癌建立的新细胞系（KU-Lu-MPPt3）中的分子改变
DOI：
10.1007/s00432-017-2541-0
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发表时间：
2018
影响因子：
3.6
通讯作者：
Satoh Yukitoshi
中科院分区：
文献类型：
--
作者：
Matsuo Yukiko;Shiomi Kazu;Sonoda Dai;Mikubo Masashi;Naito Masahito;Matsui Yoshio;Yoshida Tsutomu;Satoh Yukitoshi
目的肺微乳头状腺癌（MPP）与无MPP的乳头状腺癌相比，具有血管侵犯更频繁、更明显、发生率更高、淋巴结转移更早、预后更差的特点。在这里，我们建立了一个新的肺癌细胞系具有micropapillary structure.MethodsA 73岁的从不吸烟的日本女性，提出了一个异常的胸部阴影，被诊断为临床T2 aN 0 M0 IB期肺腺癌，并接受左上叶切除纵隔淋巴结清扫。病理学研究显示T2 aN 2 M0 IIIA期微乳头状腺癌。从新鲜切除的肺组织中获得肿瘤细胞，并用于建立KU-Lu-MPPt 3细胞系.ResultsThe KU-Lu-MPPt 3细胞在相同的培养条件下具有粘附单层、粘附簇和无粘附的悬浮簇的特征。细胞角蛋白、上皮细胞粘附分子、E-钙粘蛋白、粘蛋白-1、甲状腺转录因子-1、波形蛋白和抗程序性死亡配体1呈阳性。异种移植瘤清楚地表现出微乳头状结构。对原发瘤组织和KU-Lu-MPPt 3细胞的表皮生长因子受体基因进行序列测定和片段分析，发现其第19外显子存在E746-A750的读框内缺失。结论该细胞系为研究肺腺癌的分子生物学机制提供了一个新的模型系统，可用于肿瘤扩散的研究，并可用于分子靶向治疗和免疫治疗的研究。
PurposeLung adenocarcinomas with a micropapillary pattern (MPP) are characterized by more frequent and pronounced vascular invasion, higher incidence and more advanced lymph node involvement and poorer prognosis than papillary adenocarcinomas without an MPP. Here we established a new lung cancer cell line featuring micropapillary structure.MethodsA 73-year-old never-smoker Japanese female, presenting with an abnormal chest shadow, was diagnosed with a clinical T2aN0M0 Stage IB lung adenocarcinoma and underwent left upper lobectomy with mediastinal lymph node dissection. Pathological study demonstrated a T2aN2M0 Stage IIIA micropapillary adenocarcinoma. Tumor cells were obtained from freshly resected lung material and used to establish the KU-Lu-MPPt3 cell line.ResultsThe KU-Lu-MPPt3 cells featured adherent monolayers, adherent tufts, and suspended tufts without adhesion under the same culture conditions. The cells were positive for cytokeratin, epithelial cell-adhesion molecules, E-cadherin, mucin-1, thyroid transcription factor-1, vimentin, and anti-programmed death ligand 1. Xenograft tumors clearly demonstrated micropapillary structures. Sequencing and fragment analysis of the epidermal growth factor receptor in the primary tumor tissue and KU-Lu-MPPt3 cells revealed an in-frame deletion E746-A750 in exon 19.ConclusionsThis cell line represents a new model system for molecular studies of lung adenocarcinoma which may be suitable for investigation of cancer spread and also for development of molecular-targeting and immunotherapies, both in vitro and in vivo.