Evaluation of intervertebral disc regeneration with implantation of bone marrow mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits
Evaluation of intervertebral disc regeneration with implantation of bone marrow mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits
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使用定量 T2 图谱评估植入骨髓间充质干细胞 (BMSC) 的椎间盘再生：一项兔子研究
DOI：
10.1007/s00264-014-2481-0
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发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Shi, Rui
中科院分区：
文献类型：
--
作者：
Cai, Feng;Wu, Xiao-Tao;Shi, Rui
本研究旨在探讨骨髓间充质干细胞(BMSCs)移植对椎间盘再生的疗效，并探讨定量T2标记法评价BMSCs植入后髓核修复的可行性。方法新西兰大白兔48只，采用纤维环刺激法建立腰椎间盘退变模型，随机分为4组，即2周后将BMSCs或磷酸盐缓冲液(PBS)植入退变盘内(BMSCs组和PBS组)。未植入BMSCs或PBS的手术兔为假手术组，未手术的兔为对照组。术后2、6、10周，用磁共振成像(MRI 3.0：T)测量T2值和椎间盘高度指数(DHI)，用实时定量逆转录聚合酶链式反应(RT-PCR)检测退变椎间盘中II型胶原(COL2)和聚集素(Acan)基因的表达。髓核的T2值与Acan或COL2的表达进行回归分析。结果在退变的腰椎间盘中可观察到细胞团、纤维组织紊乱、板层间糖胺聚糖(GAG)基质和血管形成。BMSCs可存活于髓核内，并可分化为表达COL2和Acan的髓核样细胞。移植后10周，COL2和Acan基因表达增强，T2信号强度和T2值增加。BMSCs组的DHI下降较PBS组和Sham组慢。髓核内Acan、COL2基因表达与T2值呈正相关。结论骨髓间充质干细胞移植可促进退变髓核再生。定量、无创性T2图可用于评价髓核再生情况，具有较好的敏感性。
PurposeThe aim of the study was to investigate the curative effects of transplantation of bone marrow mesenchymal stem cells (BMSCs) on intervertebral disc regeneration and to investigate the feasibility of the quantitative T2 mapping method for evaluating repair of the nucleus pulposus after implantation of BMSCs.MethodsForty-eight New Zealand white rabbits were used to establish the lumber disc degenerative model by stabbing the annulus fibrosus and then randomly divided into four groups, i.e. two weeks afterwards, BMSCs or phosphate-buffered saline (PBS) were transplanted into degenerative discs (BMSCs group and PBS group), while the operated rabbits without implantation of BMSCs or PBS served as the sham group and the rabbits without operation were used as the control group. At weeks two, six and ten after operation, the T2 values and disc height indices (DHI) were calculated by magnetic resonance imaging (MRI 3.0 T), and the gene expressions of type II collagen (COL2) and aggrecan (ACAN) in degenerative discs were evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR). T2 values for the nucleus pulposus were correlated with ACAN or COL2 expression by regression analysis.ResultsCell clusters, disorganised fibres, interlamellar glycosaminoglycan (GAG) matrix and vascularisation were observed in lumber degenerative discs. BMSCs could be found to survive in intervertebral discs and differentiate into nucleus pulposus-like cells expressing COL2 and ACAN. The gene expression of COL2 and ACAN increased during ten weeks after transplantation as well as the T2 signal intensity and T2 value. The DHI in the BMSCs group decreased more slowly than that in PBS and sham groups. The T2 value correlated significantly with the gene expression of ACAN and COL2 in the nucleus pulposus.ConclusionsTransplantation of BMSCs was able to promote the regeneration of degenerative discs. Quantitative and non-invasive T2 mapping could be used to evaluate the regeneration of the nucleus pulposus with good sensitivity.