Hierarchically Nanostructured Hybrid Platform for Tumor Delineation and Image-Guided Surgery via NIR-II Fluorescence and PET Bimodal Imaging
Hierarchically Nanostructured Hybrid Platform for Tumor Delineation and Image-Guided Surgery via NIR-II Fluorescence and PET Bimodal Imaging
复制标题
通过 NIR-II 荧光和 PET 双模成像进行肿瘤勾画和图像引导手术的分层纳米结构混合平台
DOI：
10.1002/smll.201903382
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发表时间：
2019
期刊：
影响因子：
13.3
通讯作者：
Cheng Zhen
中科院分区：
文献类型：
--
作者：
Zhang Qing;Zhou Huijun;Chen Hao;Zhang Xiao;He Shuqing;Ma Lina;Qu Chunrong;Fang Wei;Han Yanjiang;Wang Da;Huang Yuanjian;Sun Yueming;Fan Quli;Chen Yue;Cheng Zhen
第二近红外窗口（NIR‐II）和正电子发射断层扫描（PET）的双模态成像具有互补优势，对肿瘤诊断和管理具有重要意义。开发具有高荧光亮度的NIR-II/PET双峰探针仍然具有挑战性。在本文中，生物启发纳米材料（黑色素点、介孔二氧化硅纳米颗粒和支撑的脂质双层）、NIR-II染料CH-4 T和PET放射性核素64 Cu被整合到混合NIR-II/PET双峰纳米探针中。所得的纳米探针表现出有吸引力的性质，如高度均匀的可调尺寸，有效的有效载荷封装，高稳定性，稳定性和生物相容性。有趣的是，将CH-4 T掺入纳米颗粒导致4.27倍的荧光增强，从而使体外和原位的体模成像更明亮。受益于荧光增强，使用纳米探针进行NIR-II成像，以精确描绘和切除肿瘤。此外，纳米探针成功地应用于肿瘤PET成像，显示了纳米探针在肿瘤中的积累，在注射后2至24小时具有清晰的对比度。总体而言，这种分层纳米结构平台能够显著增强NIR-II染料的荧光亮度，通过NIR-II/PET成像检测肿瘤，并指导术中切除。NIR-II/PET双峰纳米探针在敏感的术前肿瘤诊断和精确的术中图像引导手术方面具有很高的潜力。
Bimodal imaging with fluorescence in the second near infrared window (NIR‐II) and positron emission tomography (PET) has important significance for tumor diagnosis and management because of complementary advantages. It remains challenging to develop NIR‐II/PET bimodal probes with high fluorescent brightness. Herein, bioinspired nanomaterials (melanin dot, mesoporous silica nanoparticle, and supported lipid bilayer), NIR‐II dye CH‐4T, and PET radionuclide64Cu are integrated into a hybrid NIR‐II/PET bimodal nanoprobe. The resultant nanoprobe exhibits attractive properties such as highly uniform tunable size, effective payload encapsulation, high stability, dispersibility, and biocompatibility. Interestingly, the incorporation of CH‐4T into the nanoparticle leads to 4.27‐fold fluorescence enhancement, resulting in brighter NIR‐II imaging for phantoms in vitro and in situ. Benefiting from the fluorescence enhancement, NIR‐II imaging with the nanoprobe is carried out to precisely delineate and resect tumors. Additionally, the nanoprobe is successfully applied in tumor PET imaging, showing the accumulation of the nanoprobe in a tumor with a clear contrast from 2 to 24 h postinjection. Overall, this hierarchically nanostructured platform is able to dramatically enhance fluorescent brightness of NIR‐II dye, detect tumors with NIR‐II/PET imaging, and guide intraoperative resection. The NIR‐II/PET bimodal nanoprobe has high potential for sensitive preoperative tumor diagnosis and precise intraoperative image‐guided surgery.