ELECTROPHORETIC HETEROGENEITY AND INTERSTRAIN VARIATION OF THE LIPOPOLYSACCHARIDE OF HEMOPHILUS-INFLUENZAE
ELECTROPHORETIC HETEROGENEITY AND INTERSTRAIN VARIATION OF THE LIPOPOLYSACCHARIDE OF HEMOPHILUS-INFLUENZAE
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DOI：
10.1093/infdis/148.3.492
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
INZANA, TJ
中科院分区：
文献类型：
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作者：
INZANA, TJ
从50株H.用快速微量法检测流感病毒，并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和银染法进行分析。通过该方法分离的LPS在色谱上与常规纯化的LPS无法区分，但缺乏指示O-侧链重复单元可变聚合的大程度异质性;凝胶中出现1-4条带。H.流感病毒B型在菌株间存在差异，可用于亚型分型。根据条带迁移率的稳定性，将33株B型菌株分为11个亚型。某些菌株相同的外膜蛋白亚型不同的LPS亚型。非b型H.流感病毒与B型流感病毒在微观异质性方面基本相似，而无法分型的流感病毒通常表现出较少的电泳变异。
Lipopolysaccharide (LPS) was extracted from 50 isolates of H. influenzae by a rapid micromethod and was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Ag staining. LPS isolated by this method was electrophoretically indistinguishable from conventionally purified LPS but lacked the large degree of heterogeneity indicative of variable polymerization of O-side-chain repeating units; 1-4 bands appeared in gels. Electrophoretic profiles of LPS from H. influenzae type b varied among strains and were useful for subtyping. On the basis of stable band mobilities, 33 isolates of type b were classified into 11 subtypes. Certain isolates of identical outer-membrane-protein subtype differed in LPS subtype. The LPS profiles of non-b, typable strains of H. influenzae were generally similar in microheterogeneity to those of type b strain; those of untypable isolates usually displayed less electrophoretic variation.