Iodoacetic acid inhibition of calcium-dependent potassium efflux in red blood cells.
Iodoacetic acid inhibition of calcium-dependent potassium efflux in red blood cells.
复制标题
碘乙酸抑制红细胞中钙依赖性钾流出。
DOI：
10.1152/ajpcell.1985.248.5.c419
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Plishker,GA
中科院分区：
文献类型：
--
作者：
Plishker,GA
代谢抑制剂碘乙酸（IAA）通常用于增加红细胞中钙依赖性的钾外排。据认为，IAA的这种作用涉及对甘油醛-磷酸脱氢酶（EC 1.2.1.12）的不可逆抑制，导致细胞能量消耗。没有能量，主动运输停止，钾的损失通过增加细胞钙和阻止钾的再吸收而增加。目前的研究表明，除了这种代谢作用，增加钙依赖的钾外排，IAA也抑制这种外排。这种抑制是不可逆的，与细胞的ATP或Ca浓度无关。特定蛋白带的羧甲基化与IAA对钾外排的抑制有关。
The metabolic inhibitor, iodoacetic acid (IAA), has commonly been used to increase Ca-dependent K efflux in red blood cells. It is thought that this effect of IAA involves the irreversible inhibition of glyceraldehyde-phosphate dehydrogenase (EC 1.2.1.12), resulting in the energy depletion of the cell. Without energy, active transport stops, and the K loss is enhanced both by increasing cellular Ca and by preventing K reuptake. The present study shows that in addition to this metabolic effect, which increases Ca-dependent K efflux, IAA also inhibits this efflux. This inhibition is irreversible and is not related to the ATP or Ca concentrations of the cells. The carboxymethylation of a specific protein band correlates with IAA inhibition of K efflux.