Genetic introgression by the rose bitterling, Rhodeus ocellatus ocellatus, into the Japanese rose bitterling, R-o. kurumeus (Teleostei: Cyprinidae)
Genetic introgression by the rose bitterling, Rhodeus ocellatus ocellatus, into the Japanese rose bitterling, R-o. kurumeus (Teleostei: Cyprinidae)
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DOI：
10.2108/zsj.18.1027
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发表时间：
2001-09-01
影响因子：
0.9
通讯作者：
Kanoh, Y
中科院分区：
文献类型：
--
作者：
Kawamura, K;Ueda, T;Kanoh, Y
日本玫瑰花鱼Rhodeus ocellatus kurumeus是一种濒临灭绝的鲤科鱼类。R. O. kurumeus被认为是由于该亚种与R. O.从中国引进的眼斑蝶。利用线粒体DNA的RPLP分析和基因组DNA的RAPID-PCR分析，对该菌的遗传渐渗进行了研究。o，ocellatus转化为R. O. kurumeus。用6种限制性内切酶在线粒体DNA的D环区将这两个亚种区分开来。罗得岛岛kurumeus有11个亚种特异的RAPID标记，R. O. ocellatus有两个。除了一些R。O. kurumeus种群中，大部分R.日本的ocellatus是杂交种，分别配备了两个亚种的亚种特异性RAPID标记。然而，这些标记在杂交后代中的遗传构成在种群间差异很大。中华民国O.除两个种群的mtDNA为两个亚种的mtDNA外，其它种群的mtDNA主要分布在杂交种群中。从形态和生理性状的遗传优势来看，黑穗醋栗的遗传优势度较高。O. ocellatus对R. O. kurumeus，杂种可能具有与R. O. ocellatus对R. O. kurumeus。因此，认为R. O. kurumeus不仅遗传特性因与R. O. ocellatus，但也被R. O.眼斑鱼和杂交鱼线粒体DNA和基因组的置换。O. kurumeus与R. O.杂交种与红蜘蛛回交可加速红蜘蛛在杂交中的发育。O.眼状肌
The Japanese rose bitterling, Rhodeus ocellatus kurumeus, is an endangered cyprinid species. The main reason of drastic diminution of R. o. kurumeus has been suggested to be due to hybridization between this subspecies and R. o. ocellatus introduced from China. Both RPLP analysis of mitochondrial DNA and RAPID-PCR analysis of genomic DNA were performed to elucidate the genetic introgression of R. o, ocellatus into R. o. kurumeus. The two subspecies were distinguished in the D-loop region of mtDNA by six restriction endonucleases. Rhodeus o. kurumeus had eleven subspecies-specific RAPID markers and R. o. ocellatus had two. Except some R. o. kurumeus populations, most of the populations of R. ocellatus in Japan were hybrids, equipped with subspecies-specific RAPID markers for the two subspecies, respectively. The genetic constitution of these markers in hybrids, however, greatly differed among populations. The R. o. ocellatus mtDNA was predominantly observed in hybrid populations, except two populations with mtDNAs of the two subspecies. Judging from the genetic dominance of morphological and physiological characters of R. o. ocellatus against R. o. kurumeus, hybrids probably have the same ecological dominance as R. o. ocellatus against R. o. kurumeus. Therefore, it is considered that R. o. kurumeus not only has its genetic property spoiled by hybridization with R. o. ocellatus, but also is expelled by R. o. ocellatus and hybrids. The replacement of mtDNA and genomes of R. o. kurumeus with those of R. o. ocellatus in hybridization might be accelerated by the backcross between hybrids and R. o. ocellatus.